Transepithelial electrical resistance (TEER) has emerged as a powerful tool in ophthalmology research and has become a standard method for study of retinal pigment epithelial (RPE) cell biology and the development of these cells as a therapeutic for retinal diseases such as Age-Related Macular Degeneration (AMD). The application of TEER in assessing the barrier function and integrity of RPE cell layers has significantly advanced our understanding of retinal diseases and paved the way for innovative therapeutic interventions including some of the most promising cell therapies to date. This article explores the use of TEER in ophthalmology research, with a focus on studies utilizing RPE cells for both research and clinical applications.

EVOM™ Auto, with both 24 and 96 HTS multiwell plate capability, is a high throughput screening (HTS) platform offering fast, noninvasive sample scanning by comparing electrical resistance measurements (transepithelial/transendothelial electrical resistance: TEER). TEER measurement experiments are simple to set up and less time consuming than more complex molecular studies. TEER measurement can be used as the primary scanning method to identify any physiological changes that can then be further evaluated by other methods. EVOM™ Auto can capture TEER measurements in 24 and 96 HTS multiwell plates from Corning, Millipore, or MatTek. The sample preparation time in these HTS plates is efficient, allowing for fast, multi-channel pipetting options.

The EVOM™ Auto autosampler ships with a red electrode array lock in place to prevent the electrode array connection port from moving during transit. Here you can see how to easily remove it without damaging the lock or the autosampler.

Below are some popular pullers from WPI, including Sutter pullers. Here we compare them so you can choose a system that is best suited for your application.

The WPSS01 skin stapler is an affordable, reliable solution for veterinary wound closures. A skin stapler is designed for the efficient closure of surgical incisions or wounds by applying surgical staples to the skin, reducing surgery time and time under anesthesia. Each disposable skin staple cassette contains at least thirty-five (35) 316L stainless steel staples.

Each reusable skin stapler handle is provided sterile with one disposable staple cassette already loaded into the handle. Discard the disposable staple cassette after the procedure. The reusable handle may be cleaned and resterilized (autoclave, EO) for subsequent procedures and will effectively deploy at least twenty (20) disposable staple cassettes and withstand at least twenty (20) autoclave cycles.

Surgical forceps are available in several different grades, including disposable, standard stainless steel, German steel, Swiss made, and titanium. Your choice of instrument grade will depend on your requirements and your budget. Here are some considerations to help you make an informed decision.

Choosing a dependable lab equipment supplier is crucial for ensuring the quality, reliability, and timely delivery of the equipment you require.  When your research is on the line, you need a company you trust, a supplier with a proven track record and a service team to back you up. Here are a few important considerations when selecting a lab equipment supplier.

Reducing hand fatigue while using surgical scissors is important, not only for your comfort, but also to maintain the precision of your work. Here are some tips to help reduce strain and minimize hand fatigue.

Surgical forceps are available in several different grades, including disposable, standard stainless steel, German steel, Swiss made, and titanium. Your choice of instrument grade will depend on your requirements and your budget. Here are some considerations to help you make an informed decision.

Surgical forceps and surgical tweezers are handheld surgical instruments for grasping or clamping, but they serve different purposes and have distinct features. While both are used for manipulating small objects, forceps are typically used in medical and research environments, and tweezers have a broader use in hobbies, daily routine tasks, and mechanical applications. Here we will explore some of the differences between the two, including their construction and uses.

Dumont is a well-known brand that specializes in the production of high-quality precision forceps and tweezers for surgical and scientific use, as well as for electronics, jewelry, and other  applications. Dumont surgical instruments, particularly Dumont tweezers, are highly regarded for their precision, craftsmanship, and durability.

Metzenbaum and Mayo scissors are both types of surgical scissors, and while they may look similar at first glance, they serve different purposes. Let’s look at the differences between the two scissors styles.

Let’s take a look at how to set up a Digital Rat/Mouse, Neonatal Rat Stereotaxic frame. In a few steps you will be up and running.

SARASOTA, Fla., November 10, 2023 /PRNewswire/ -- World Precision Instruments (WPI), a leading provider of high-quality laboratory instruments and equipment for basic research and drug development, is proud to announce the establishment of its Scientific Advisory Board (SAB). Comprised of renowned experts in various scientific disciplines, the SAB will play a crucial role in guiding and advancing WPI's commitment to innovation and excellence in scientific research.

SARASOTA, Fla., October 23, 2023 /PRNewswire/ -- World Precision Instruments (WPI) is excited to expand the capability of the EVOM™ Auto, now offering both 24 and 96 multiwell capacity in one single platform.  The EVOM™ Auto enables rapid and reproducible measurements of Transepithelial Electrical Resistance (TEER), a well-established, non-invasive and widely accepted method of evaluating epithelial and endothelial cells and tissues. 

Transepithelial/transendothelial electrical resistance (TEER) measurement is one of the most used practices to evaluate cellular health, such as cellular confluence, barrier integrity, or barrier function of cellular monolayers grown onto multiwells. The TEER measurement using WPI’s Epithelial Voltohmmeter (EVOM) is considered the gold standard because of its reliable measurements and numerous literature citations using various cell types. The EVOM™ Manual and EVOM™ Auto along with different choices of electrodes (STX4, STX HTS high throughput screening, EndOhm chambers, and multielectrode array for EVOM™ Auto) allow researchers to perform and analyze cell samples in 6, 12, and 24 removable inserts and 24 and 96 HTS multiwell plate formats. The major challenges researchers may encounter while performing studies to capture TEER measurement include:
·      Unstable read outs
·      Out of range values 
·      Inconsistent measurements among sample replicates or batches.
Consider the following factors to overcome any TEER measurement problems and obtain accurate and reliable measurements. 

Transepithelial Electrical Resistance (TEER) is a widely used technique in the life sciences fields and therapeutic development. It measures the electrical resistance across a cell monolayer, providing information about the integrity and functionality of epithelial barriers. TEER has proven to be a valuable tool in various fields, including drug absorption studies, tissue engineering, and disease modeling. This article aims to explore the benefits of TEER and its applications in different research areas.