Microinjection Tool Box
The system depicted includes components often favored by researchers:
- MICRO-ePUMP Pneumatic PicoPump with built in MICRO-ePORE™ Cell Penetrator to facilitate microinjections
- SU-P1000 Micropipette Puller
- M4C Stand
- M3301R Micromanipulator
- PZMTIII Microscope with Optional Lighted Base with Articulating Mirror and optional PRO-300 HDS Camera and View Screen
- E2XX Micropipette Storage Jar
- Z-MOLDS Microinjection and Transplantation Molds
- 14003-G Vannas spring scissors
- Glass Capillaries
- 77020 Glass Tweezers
- FluoroDish Optical Grade Glass Bottom Culture Dishes
VIDEO: Setting up the WPI PicoPump
Designed to simplify intracellular injection and a variety of other microinjection tasks, WPI’s PicoPumps (PV830 and PV820) use precisely regulated pressures for securing cells and injecting them with fluid. Injected volumes range from picoliters to nanoliters. Separate ports supply positive and negative pressure—positive pressure for high-pressure ejection, and suction for supporting the cell or for filling the pipette from the tip. In this quick series, you can see how to setup a PicoPump.