VIDEO: Sensor Calibration for WPI's TBR4100 Free Radical Analyzer (NO, HPO & H2S)
When using a TBR Free Radical Analyzer, accurate measurements of an analyte requires an accurate calibration. The calibration of nitric oxide, hydrogen peroxide and hydrogen sulfide sensors is similar. WPI oxygen and glucose sensors require different methods which will not be discussed here.
Benefits of Nitric Oxide Detection using the WPI Free Radical Analyzer
Nitric oxide (NO) is an essential signaling molecule and is known to play a significant role in a multitude of physiological systems including the central nervous system (CNS), the cardiovascular system, the gastrointestinal tract, the immune system, and the renal system. 1-5 However, being highly reactive, detection and quantification of NO is very difficult.6,7 It requires a sensor that is sensitive, selective to NO, and easy to calibrate.
Calibrating Your Oxygen Sensor for use with the TBR
World Precision Instruments chemist Nikki Scafa demonstrates how to calibrate your ISO-OXY-2 or OXELP oxygen sensor with the TBR4100 free radical analyzer. For more information on biosensors, see www.wpiinc.com/biosensors.
Biosensor Specifications
WPI offers a range of biosensors for monitoring nitric oxide, hydrogen peroxide, oxygen and hydrogen sulfide. Specifications for these sensors are detailed below.
How to Make PBS Buffer Solution
In this video, WPI Chemist Nikki Scafa demonstrates how to make a PBS buffer solution for use with WPI microsensors.
How to Make 0.1M Copper II Chloride
In this video, WPI Chemist Nikki Scafa demonstrates how to make a 0.1M solution of CuCl2 for calibrating WPI NO microsensors.
How to Fill a 2 mm Sensor Sleeve with Filling Solution
In this video, Nikki demonstrates how to prepare and fill a 2mm biosensor and prepare to calibrate an ISO-OXY-2 oxygen sensor.
How to Calibrate an ISO-HPO100
In this video, WPI Chemist Nikki Scafa demonstrates how to calibrate the WPI ISO-HPO100 hydrogen peroxide microsensor.