Improve Research Results with Glass Bottom Culture Dishes
Get the highest quality images and video for your research with FluoroDish™ Cell Culture dishes. Their optical quality glass bottom is as thin as a coverslip, which minimize the degree of distortion and excellent heat transfer without any of the autofluorescence issues so common with plastic petri dishes.
WPI offer three different styles in various sizes to suit your application. For live cell imaging, embryo research, and life science researchers working with small sample volumes, the 35mm FluoroDish™ with a 10mm well (FD3510) is ideal. Researchers working with expensive chemicals or experimental drugs have chosen the FD3510 dish as an ideal tool in their research. They are also an excellent choice for microinjection applications, because they are designed with the lowest access angle for easier insertion of a micropipette during cellular microinjection. FluoroDish™ is also available in 35mm (FD35) or 50mm (FD5040) sizes for cell culturing applications.
WPI also offer coated dishes that are useful in promoting cell adhesion to culture surface. 35mm dishes coated with Collagen, Fibronectin, Poly-D-Lysine, Poly-L-Lysine, or Vitronectin are available.
Cited in 575 reference articles (in the NIH PubMed Central® alone), our optical grade, glass bottom culture dishes are unique in the marketplace and conform to strict quality control standards.
Optical Grade Glass Bottom for Exceptional Imaging Quality
The FluoroDish™ bottom glass has superior UV transmission (30% transmission at 300 nm, compared to less than 7% for the most popular German glass). Stringent quality control ensures that glass thickness of the dish bottom is 0.17 ±0.01 mm. That is as thin as a coverslip. The thin glass lets you use a higher magnification, so you get the highest possible image resolution. Glass avoids the problems of inherent autofluorescence present in plastics and yields a higher signal-to-noise ratio by avoiding interference.
Specially Formulated Low Toxicity Adhesive
FluoroDish™ petri dishes use a specially formulated adhesive that is optically clear, durable and with extremely low toxicity. Tests by an independent laboratory have shown that the 96-hour survival rate of rat embryos typically exceeds 95% when kept in a FluoroDish™. Test results are available on the product page.
Benefits
When you need quality glass bottom cell culture dishes with less optical distortion, excellent UV transmission, low cytotoxicity and guaranteed sterility, order WPI Fluorodish™.
- Optical quality glass bottom for shorter working distances, larger numerical aperture and higher magnification
- Non-fluorescent glass so you can discern weaker signals
- Allows the use of immersion objectives
- Flat bottom optimizes heat exchange
- Less optical distortion and superior UV transmission
- Low cytotoxicity adhesive to ensure cells' survival
- Individually packaged and gamma sterilized
- Multiple sizes and designs to suit your application
- Coated dishes available for better cell adhesion
- Blackwall dishes are available to block stray lights and reduce background noise.
- Different sizes designed for low volumes or large growth areas
Applications
WPI’s FluoroDish™ cell culture dishes provide exceptional imaging quality for many applications requiring the use of inverted microscopes such as:
- High resolution live cell imaging analysis
- Microinjection
- Electrophysical recording of fluorescent-tagged cells
- Cell adhesion assays
- Promotion of cell attachment and spreading.
Taking advantage of WPI’s extensive experience with low toxicity adhesives, FluoroDish™ use a specially formulated adhesive that is optically clear, durable and with extremely low toxicity. Tests by an independent laboratory have shown that the 96-hour surviving rate of embryos is 100% when kept in FluoroDish™, substantially better than other brands. The bottom glass has superior UV transmission (30% transmission at 300 nm, compared to less than 7% for the most popular German glass). Stringent quality control ensures that glass thickness is 0.17 ±0.01 mm.
Conventional plastic petri dishes and chambers limit the use of the inverted scope, because the thick plastic bottom requires a long working distance objective available only in lower magnifications. Each WPI dish has a flat (0.17mm thick), optical quality glass bottom, allowing the use of a much shorter working distance, larger numerical aperture (NA) and higher magnification (up to 100X). The larger NA and higher magnification provide superior quality imaging for both classical and fluorescence microscopy. Higher effective NA yields brighter images for fluorescence and higher resolution in image analysis. The glass bottom permits the use of immersion objectives with media such as water, glycerin or oil for the highest magnification possible. To optimize heat-exchange, WPI’s glass-bottom dish is designed to be flush (flat) with the microscope stage or heating unit, eliminating the air gap that exists with modified plastic dishes where a cover slip was inserted.
Multiple sizes
We have a 50 mm diameter FluoroDish™ and two types of 35 mm diameter FluoroDish™. An inner well is created within the dish by the glass bottom and the tissue culture grade polystyrene which forms the sides of the dish. They are individually packaged and gamma sterilized.
The 35 mm FluoroDish™ has outside dimensions similar to that of a Corning 35 mm petri dish and has 23.5 mm diameter glass window (FD35) or 10 mm diameter glass window (FD3510). Most heaters and perfusion adapters designed for the Corning 35 mm petri dish also fit this petri dish.
Coated or uncoated dishes
The 23 mm well FluoroDish™ are available uncoated or coated. Some cell lines (like the PC3 and HEK) adhere well to the uncoated glass bottom dish. The coated FluoroDish™ improves the adhesion of neuron cells. WPI offers 35mm FluoroDish™ available coated with Collagen, Fibronectin, Poly-D-Lysine, Poly-L-Lysine, or Vitronectin. The coated dishes are stored in applicable environment (10°C) for longevity of the coating.
Low volume dishes or large growth areas
The 10 mm glass dish (FD3510) has low sidewalls for easy microelectrode access and low solution volume. The low microelectrode access angle is the lowest among all of 35 mm dishes on the market (very close to that of a 50 mm dish). The dish needs only about 115 µL to cover the bottom well, an important feature when using expensive drugs and chemicals.
The 50 mm petri dish (FD50) has a large growth area (35 mm well diameter), a low access angle for microelectrodes, and grips for easy handling.
Features
- The 35mm FluoroDish™ has outside dimensions similar to that of a Corning 35 mm petri dish and has 23.5 mm diameter glass window. Most heaters and perfusion adapters designed for the Corning 35mm dish will also fit this FluoroDish™. Certain types of cell lines, like the PC3 and HEK, adhere well to the uncoated glass bottom petri dish. You can also apply to the uncoated dish any special coating that is best for your cell line.
- The 50 mm cell culture dish has a large growth area (35 mm well diameter), a low access angle for microelectrodes, and grips for easy handling.
NOTE: Due to supply constraint, this product is currently non-returnable/non-refundable. For details, please refer to the WPI Terms & Conditions.
