How to Properly Mix a Burstable Sylgard Twin Pack
Sylgard is a 2-part silicone elastomer with a very low dielectric constant, which makes it ideal for patch clamping and many other lab applications, especially in electrophysiology. The twin pack is a convenient, single use sleeve containing both parts of the adhesive. We will show you how to properly mix the Sylgard Twin Pack.
VIDEO: How to Remove EVOM™ Auto Electrode Array Lock
EVOM™ Auto, with both 24 and 96 HTS multiwell plate capability, is a high throughput screening (HTS) platform offering fast, noninvasive sample scanning by comparing electrical resistance measurements (transepithelial/transendothelial electrical resistance: TEER). TEER measurement experiments are simple to set up and less time consuming than more complex molecular studies. TEER measurement can be used as the primary scanning method to identify any physiological changes that can then be further evaluated by other methods. EVOM™ Auto can capture TEER measurements in 24 and 96 HTS multiwell plates from Corning, Millipore, or MatTek. The sample preparation time in these HTS plates is efficient, allowing for fast, multi-channel pipetting options.
The EVOM™ Auto autosampler ships with a red electrode array lock in place to prevent the electrode array connection port from moving during transit. Here you can see how to easily remove it without damaging the lock or the autosampler.
EVOM™ Auto: Fix Software Switching Modes
If the EVOM™ Auto software switches from Resistance to TEER mode while you are selecting menu options, here's a quick tip:
Be careful when selecting Resistance mode, do not tap the background window and do not click on the side arrows.
Tapping the background window will switch you to TEER mode.
Instead, tap an option on the forefront square window to stay in Resistance mode.
VIDEO: Quick Reference for Sterilization of Surgical Instruments
After a good cleaning and disinfection, your surgical instruments may be sterilized using a variety of methods. The chart below shows two common methods, autoclaving and cold sterilization. Sterilization destroys all microbial life. Some chemical sterilants can be used as high level disinfectants (HLD) when used for shorter exposure periods.
VIDEO: Using the Adaptive Mode on the ATC2000
The ATC2000 is a low noise heating system for maintaining animal body temperature during expirimental procedures. Here we show you how to use the adaptive mode on the ATC2000 system.
VIDEO: How to Take Voltage Measurements with the EVOM™ Manual
EVOM™ Manual is WPI’s newest instrument to measure Trans Epithelial Electrical Resistance (TEER). Let’s look at how to set it up and take voltage measurements.
VIDEO: 9 Reasons to Trust the EVOM™ Manual TEER Measurement Meter
WPI’s EVOM™ technology is the gold standard for delivering stable and repeatable Trans Epithelial Electrical Resistance (TEER) measurements. Here are nine reasons to trust the new EVOM Manual TEER Meter for qualitatively measuring cell monolayer health and quantitatively measuring cell confluence.
VIDEO: How to Take Resistance Measurements with EVOM™ Manual
EVOM™ Manual is WPI’s newest instrument to measure Trans Epithelial Electrical Resistance (TEER). Let’s look at how to set it up and take resistance measurements.
VIDEO: Which EVOM™ Electrode is Right for Your Application?
WPI’s EVOM™ Manual is the gold standard for delivering stable and repeatable Trans Epithelial Electrical Resistance (TEER) measurements. The EVOM™ Manual is the newest TEER meter. It qualitatively measures cell monolayer health and quantitatively measures cell confluence by determining an increase or a plateau in tissue resistance detected using our innovative EVOM™ technology. WPI’s state of the art EVOM™ technology provides you with real-time, valuable feedback during experimental measurements.
VIDEO: How to Replace the STX4 Electrode Blades
The new STX4 electrode works with the EVOM3, and the blades are replaceable. EVOM3 is used for making TEER (TER) Measurements in cell culture plates. Here's a quick tutorial to show you how to replace the blades.
VIDEO: Understanding the ATC2000 Modes of Operation
The ATC2000 is a low noise heating system for maintaining animal body temperature during experimental procedures. It has three modes of operation, which we discuss here.
VIDEO: How to Reset the Factory Defaults on the ATC2000
The ATC2000 is a low noise heating system for maintaining animal body temperature during experimental procedures. Here we show you how to reset the factory defaults on your ATC2000.
VIDEO: Troubleshooting the ATC2000
The ATC2000 is a low noise heating system for maintaining animal body temperature during expiremental procedures.
VIDEO: How Does the PID Control Work in an ATC2000?
The ATC2000 is a low noise heating system for maintaining animal body temperature during experimental procedures.
VIDEO: How to Set the Configuration Parameters for the ATC2000
ATC2000 is a low noise heating system for maintaining animal body temperature during experimental procedures.
VIDEO: How to Set the Controlled Temperature for the ATC2000
ATC2000 is a low noise heating system for maintaining animal body temperature during experimental procedures.
VIDEO: How to Set Up WPI's Animal Temperature Controller
ATC2000 is a low noise heating system for maintaining animal body temperature during experimental procedures. The temperature controller provides electrically quiet control over the heating plate, so that you can place the animal directly onto the heating plate. Here’s how to set up the system.
VIDEO: WPI's Top 5 Needle Holders
Needle holders, also known as a needle drivers. They are used in wound closure, ligation, reanastomosis and other surgical procedures which require suturing. The needle is usually held in position by a clamp mechanism, which allows the operator to manipulate the needle through various tissues. The jaws are generally textured and short in comparison to the shank to retain a solid grasp on the needle.
VIDEO: How to Understand the ATC2000 Display
ATC2000 is a low noise heating system for maintaining animal body temperature during experimental procedures. Here we will look at the main display on the front panel to see what information is available and the three operational modes.
VIDEO: 5 Reasons to Choose WPI's Animal Temperature Controller
The ATC 2000 is a low noise heating system for maintaining animal body temperature during experimental procedures. Here are five reasons to choose the WPI Animal Temperature Controller. WPI's Adaptive control is unique in the industry, giving you precision control without the need for complex programming when it counts. Simple to setup. Stable control. No local hotspots.
VIDEO: Guide for Ultrasonic Cleaning of Surgical Instruments
Ultrasonic cleaning is the most effective cleaning method for maintaining your surgical instruments, because of its cavitation. Vibrating sound waves create micron-size bubbles in solution that grow as the pressure in the unit changes.
VIDEO: Stain Guide for Surgical Instruments
Although stainless steel is corrosion-resistant, it can still rust and stain if it is handled improperly. To determine if a discoloration is rust or just a stain, erase the discoloration with a pencil eraser. If there is pitting in the metal under the discoloration, it is corrosion. If the discoloration is removed, it was just a stain.
VIDEO: Comparison of Metal for Surgical Instruments
Surgical instruments are designed to perform diagnostic, therapeutic, or investigative operations having specific functions such as to cut or incise, retract, grasp, hold or occlude, dilate or probe, suture or ligate.
VIDEO: Digital Microforge: Setting up the Microforge Controller
The DMF1000 is a microprocessor-controlled microforge designed for fabrication of both small patch clamp glass pipettes and larger injection pipettes. The DMF1000 can be used for polishing patch clamp pipettes, microforging holding pipettes, microforging beveled injection pipettes, and for calibrating pipette tips for microinjection. Here we will show you how to configure the DMF1000 controller.