Seven Tips for Avoiding Common Cell Culture Dish Mistakes
Petri cell culture dishes like WPI’s FluoroDishes™ are commonly used in laboratories, but they require precision and care to ensure the accurate results of your work. Let’s look at several common mistakes made with cell culture dishes and how you can avoid them.
Different Types of Cells Cultured in Laboratories
The foundation of many research laboratories is the ability to do in vitro studies, which rely on the ability to culture cells in the laboratory. Cell culture technologies have advanced significantly, allowing researchers to successfully culture many types of cell lines.
Significance of Transepithelial/ Transendothelial Electrical Resistance (TEER) Measurement in Lung In vitro Models
Epithelial and endothelial cells are known for their barrier function like selective permeability. Lung tissue is comprised of epithelial cellular layers, residing adjacent to endothelial layers and allowing exchange of oxygen and carbon dioxide between lung and blood. Currently, the biomedical research community’s major focus area is to understand the details of the infection caused by this novel coronavirus (2019-nCoV) or by similar viruses. This new virus can disrupt the lung’s normal cleaning ability to get rid of foreign particles in the lung, and the selective permeability or barrier function of the lung may become severely impaired. The adverse physiological effect may be sustained once the inflammatory cascade is initiated.
Genetic Transfer (viral or non-viral vector) Using Microprocessor-Controlled Injector
With the first approved human gene therapy trial in 19891 (Rosenberg, et.al.), gene therapy has come a long way in modern medicine and is making inroads in clinics and the market in general.2,3 2017 was an important year for gene therapy when Luxurna, the first human gene therapy drug for an inherited retinal dystrophy, was approved by Food and Drug Administration (USA).