Proper storage and handling of petri dishes is a simple but critical part of maintaining sterility, surface integrity, and reproducible results in any research lab. Dust, humidity, scratches, or premature opening can compromise cultures long before they reach the incubator or microscope. Whether you’re using standard culture dishes, ECM-coated formats, or advanced glass-bottom dishes for imaging, following a few key best practices helps protect your samples and ensure consistent performance. This guide walks through the top tips for storing petri dishes of all types, with notes for coated and glass-bottom formats that require additional care.