Microinjection Tool Box

The system depicted includes components often favored by researchers:

  1. MICRO-ePUMP Pneumatic PicoPump with built in MICRO-ePORE™ Cell Penetrator to facilitate microinjections
  2. SU-P1000 Micropipette Puller
  3. M4C Stand
  4. M3301R Micromanipulator
  5. PZMTIII Microscope with Optional Lighted Base with Articulating Mirror and optional PRO-300 HDS Camera and View Screen
  6. E2XX  Micropipette Storage Jar
  7. Z-MOLDS Microinjection and Transplantation Molds
  8. 14003-G Vannas spring scissors
  9. Glass Capillaries
  10. 77020 Glass Tweezers
  11. FluoroDish Optical Grade Glass Bottom Culture Dishes

 

Instruments for Zebrafish Research - Oocytes, Embryos, Larvae and Adults

World Precision Instruments (WPI) offers a broad range of research instruments for zebrafish (Danio Rerio) research, from the time the genetics of the subject are modified through the point where the physiological effects of the change are monitored. Whether researchers are performing microinjection or making cardiovascular measurements, the WPI product line includes instruments for research on oocytes, embryos, larvae and adult zebrafish.

Affordable New Injector: Setup the PV850 System

The PV850 Injector is designed to simplify intracellular injection and a variety of other microinjection tasks. The PV850 uses regulated air pressure for injecting cells with fluid. Injected volumes range from picoliters to nanoliters. The port supplies positive pressure for high-pressure ejection maximum of 87 PSI.

Affordable New Injector: Configure the PV850 Software

The PV850 Injector is designed to simplify intracellular injection and a variety of other microinjection tasks. The PV850 uses regulated air pressure for injecting cells with fluid. Injected volumes range from picoliters to nanoliters. The port supplies positive pressure for high-pressure ejection maximum of 8 7PSI.

Popular UMP3 Injector is Not Your Ordinary Syringe Pump

The UMP3 UltraMicroPump is easy to configure using the SMARTouch interface. It is pre-programmed for popular microsyringes and allows for user-defined syringe parameters, too. This ensures nearly universal compatibility. You can control up to two pumps, either simultaneously or independently, and the optional foot switch means hands-free operation. You can instantly verify actual injection volume on the graphical display. You can even use the touch screen while wearing gloves.

Researcher Shares Video of MICRO-ePORE™ Assisted Cell Penetration

MICRO-ePORE™ pinpoint cell penetrator is a simple and versatile system that can be used to facilitate microinjection of a diverse array of compounds and biomolecules into oocytes and pre-implantation stage mammalian embryos. Patent pending Flutter Electrode Technology assists in small, clean, precise membrane penetration without tearing or damaging the membrane. It results in substantially increased viability of embryos.

Zebrafish Microinjection using the UMP3/Micro4

Watch how researchers from the University of Chicago inject adult zebrafish using a 10μl NanoFil microsyringe controlled by a Micro4 controller and UltraMicroPump III (UMP3-1 includes one UMP3 pump and a Micro4 contro

Zebrafish Microinjection Technique from JoVE

Chiara Cianciolo Cosentino, at the University of Pittsburgh, describes how she uses intravenous microinjections of zebrafish larvae to study acute kidney injury in this JoVE video. You can watch this video on JoVE. WPI equipment shown in this video includes:

Microinjection in Zebrafish Otocytes

Researchers at the University of Michigan are using WPI's PV820 for injecting a morpholino solution into the lumen of the otic vesicle of 1-day old zebrafish embryos.  Then, they use electroporation to introduce mif and mif-like morpholinos into the developing inner ear tissues.