Gas anesthesia with Isoflurane is quickly becoming the standard method of general anesthesia for rats and mice in biomedical research. The advantages of using gas anesthesia for in-vivo examinations include:

• Fewer complications than injectable agents
• Allows for longer exam duration
• Easier administration and control
• Readily incorporated into existing procedures
• Level plane of anesthesia
• Minimal animal handling/Less stress
• No controlled substances required