How to Store Petri Dishes: 10 Best Practices for Lab Sterility and Coating Integrity
Proper storage and handling of petri dishes is a simple but critical part of maintaining sterility, surface integrity, and reproducible results in any research lab. Dust, humidity, scratches, or premature opening can compromise cultures long before they reach the incubator or microscope. Whether you’re using standard culture dishes, ECM-coated formats, or advanced glass-bottom dishes for imaging, following a few key best practices helps protect your samples and ensure consistent performance. This guide walks through the top tips for storing petri dishes of all types, with notes for coated and glass-bottom formats that require additional care.
Understanding Microscope Objectives
A variety of microscope objectives are available. All objectives use lenses to focus light. Light is broken down into various wavelengths (colors) as it travels through a lens. The various wavelengths have different focal points. That means that red, green and blue appears to focus at different points. This is called chromatic aberration. Spherical aberrations are focal mismatches caused by the shape of the lens. Quality lenses are designed correct for chromatic and spherical aberration to bring the primary colors to a common focal point. These terms may help you determine the best objective for your application:
APP NOTE: Microinjection Setup 101
When it comes to setting up microinjection systems, the options appear endless. The pictures below give some broad suggestions on how you might set up your own system. Keep in mind that many parts are interchangeable depending on your needs or preferences.
Microscope Basics
Microscopes are a standard laboratory tool, but purchasing the right microscope for a particular application can be a challenge. First, consider how you will use the instrument. Are you looking at slides, dissecting a small animal or performing a surgery? (The application dictates the necessary working distance and power of magnification.) What kind of a stand will you be using? (Boom stand, articulating arm or post stand) Will the microscope be used in a classroom setting? (A trinocular scope offers the option of including a camera.) Will you need a camera? (A camera allows you to project the microscope image on a PC or TV or to take still images.) The answers to these questions help you determine the required working distance, level of magnification, type of mounting stand and hardware required.