Pneumatic PicoPump: Injection and Vacuum

• Inject into a single cell down to picoliter volumes
• Regulated Hold & Injection Pressures, and Vacuum Pressure (external pressure source required, and vacuum if needed for your application)
• Pressure Input: 0-120 PSI
• Pressure Output: 0.3-90 PSI

Benefits

• Hold pressure prevents backfilling of the pipette by capillary action
• Ultra-accurate solenoid valves to deliver ultra precise volumes, repeatedly
• PV830 includes vacuum pressure for filling pipettes from the tip or securing cells during injection

Premium Warranty Available

Applications

• Intracellular injection, transfection for gene modification (ie. knockout studies)

Components

The PV830 System includes all the components listed below.

The PV830 model provides separate regulation and pressure gauges for the eject and hold pressures. In addition to the features of the PV820, the PV830 model also includes a vacuum pressure regulator and vacuum gauge.

The optional 5430-XX PicoNozzle Kit has a Luer-fitted pipette holder for quick and easy changes. Choose your kit based on the pipette OD (XX indicates OD: 1.0, 1.2, 1.5 or 2.0 mm). The red handle has a larger diameter so that it fits easily into most manipulators. This kit contains:

(1) MPH6S microelectrode holder

(1) Handle for the MPH6S (4" hollow tube with male Luer fitting at both ends-handle diameter is 6.25 x 100 mm.)

(1) 5’ tubing (0.060" ID, 0.120" OD, male locking Luer fitting on one end and a female locking Luer fitting at the other end, rated for 200 PSI and 86 durometer shore A)

Hold Pressure Prevents Backfilling

Like the PV830, our previous Lecacy model, the PV820, was designed to simplify intracellular injection. The PV830 is the upgraded model offering the vacuum port capability. 

Each PicoPump is supplied with a 5430-ALL kit that includes two PicoNozzles and tubing to connect the holders to the pressure and vacuum ports.

Vacuum Pressure for Additional Applications

Eject pressure supplies a high-pressure pulse for injecting fluid. Hold pressure, which is not sufficient to cause fluid ejection, is used to prevent back filling of the pipette by capillary action or diffusion when the solenoid is inactive. Vacuum can be applied through the ejection port, but vacuum in the PV820 must be externally regulated. To fill pi­pettes from the tip or through a secondary port to secure a floating cell during microinjection, vacuum may be switched from regulated vacuum to at­mo­sphere by using the switch on the front panel.