World Precision Instruments chemist Nikki Scafa demonstrates how to calibrate your ISO-OXY-2 or OXELP oxygen sensor with the TBR4100 free radical analyzer. For more information on biosensors, see www.wpiinc.com/biosensors.

Micro4™ from World Precision Instruments is an easy-to-use but very capable controller for WPI's Nanoliter Injector and UMP3 UltraMicropPump.

WPI's Nanoliter 2010 Microinjection Pump is ideal for many applications, including zebrafish, xenopus oocytes and drosophila. MICRO4, an optional microprocessor-based controller, can provide an "intelligent" and easy-to-use interface to up to four Nanoliter Injectors. Operating parameters are set with the membrane key-pad and LCD display.

Watch as Barney and Kelly Boyce set up an InVivo microscope chamber. Ideal for live cell imaging, the chambers, along with heaters, carbon dioxide and oxygen controllers and stagetop environments are sold by World Precision Instruments.

Gas anesthesia with Isoflurane is quickly becoming the standard method of general anesthesia for rats and mice in biomedical research. The advantages of using gas anesthesia for in-vivo examinations include:

• Fewer complications than injectable agents
• Allows for longer exam duration
• Easier administration and control
• Readily incorporated into existing procedures
• Level plane of anesthesia
• Minimal animal handling/Less stress
• No controlled substances required

Watch how researchers from the University of Chicago inject adult zebrafish using a 10μl NanoFil microsyringe controlled by a Micro4 controller and UltraMicroPump III (UMP3-1 includes one UMP3 pump and a Micro4 contro

Chiara Cianciolo Cosentino, at the University of Pittsburgh, describes how she uses intravenous microinjections of zebrafish larvae to study acute kidney injury in this JoVE video. You can watch this video on JoVE. WPI equipment shown in this video includes:

Two-barrel, 3-barrel, 5-barrel and 7-barrel glass capillaries are available at WPI. Multi-barrel configurations are designed especially for microiontophoresis. Because the capillaries are fused together during manufacturing, you will not need to twist them while pulling to seal the tips together. An inner filament in each barrel makes filling easy and fast.

See how simple it is to operate the new ATC2000 Animal Temperature Controller. The new adaptive mode takes the guesswork out of temperature control so you can focus on the details of your own experiment.

LabScribe is the powerful software included with Lab-Trax™ (formerly called DataTrax). The acquisition and data display in LabScribe is easy and straight forward. It supports real units and a time-based display that is not coupled to sample rate. You can display up to 1,000,000 data points per channel on the screen. Scrolling, zoom-in and zoom-out tools, together with a searchable list of user interventions, make finding important areas of data easy. And, of course, you can print or export anything that you see.

WPI surgical instruments were recently featured in a JoVE video that demonstrates a new method for cross pollinating grasses.

Watch as Gabe turns a binocular surgical microscope into a trinocular microscope and adds a video camera.

The PSMB5N Surgical Microscope has a motorized focusing system that allows for hands free operation. It is lightweight, compact and easy to maneuver. Dual bulbs prevent illumination failure during surgery. It has an optional video adapter and five magnification steps. This video shows you how to assemble your microscope.

WPI now offers suction tweezers that are ideal for picking up small objects with a flat surface. The Pickup Tweezers are perfect for handling and positioning coverslips, removing small tissues from a solution or manipulation of small electronic components. The Pickup Tweezers (#504523) require no power or batteries, and are safe from all electrostatic discharge. This kit includes the metal body (handle), a straight metal needle, an angled metal needle, three rubber cups (4, 6 and 9mm diameter) and a lubricant set. When your fingers are just too big, and standard tweezers are awkward, the Pickup Tweezers are perfect.

Researchers at the University of Michigan are using WPI's PV820 for injecting a morpholino solution into the lumen of the otic vesicle of 1-day old zebrafish embryos.  Then, they use electroporation to introduce mif and mif-like morpholinos into the developing inner ear tissues.

In this video, Mike Pizza demonstrates how to front fill a Nanofil syringe using MicroFil.

Microinjection uses either a metal needle or a glass micropipette to inject small liquid volumes. For example, genetic material may be inserted into a living cell, a drug introduced into an eye or brain, or fluid injected into a muscle. Typically, microinjection is performed under a microscope. A stereotaxic setup may be required.

WPI offers a variety of pumps along with special syringes, stereotaxic frames, glass capillaries and needles. Here we will highlight our microinjection pumps. The setup you choose depends on the size of injection aliquots, the volume to be injected and the size of needle or glass tip chosen. The products described below are listed in order by the smallest volume they can inject. See the comparison chart below or the related post links at the bottom.

The barbed tubing assortment kit contains over 250 items. All the items are pictured on a grid below. The darker lines in the image grid represent centimeters, and the smaller ones are 2.5 mm apart.

Surgical Loupes help to alleviate eye strain by enlarging the image when you are working on tiny subjects or conducting precision operations. They are portable and easier to use than a surgical microscope. However, they are not created equal, and choosing the pair that's right for you is important to your satisfaction.

Crafting high-quality glass micropipettes is essential for precision work in microinjection, patch clamping, electrophysiology, and a wide range of microsurgical and research applications. WPI offers a complete suite of microforges, pullers, and bevelers designed to help researchers create consistent, reproducible pipette shapes tailored to their experimental needs. Whether you’re pressure-polishing patch-clamp pipettes, preparing multi-barrel assemblies, or measuring electrode impedance, the tools below provide reliable control, advanced programmability, and robust optical performance to support even the most demanding labs.

Cuvettes come in a variety of shapes and sizes, but one of the most important specifications of a cuvette is its Z-dimension. The Z-dimension of an instrument (cuvette holder or spectrometer) is the distance from the bottom of the cuvette chamber floor to the center of its light beam (see image). A cuvette’s Z-dimension must match the Z-dimension of the instrument with which it will be used.