Nanoliter 2020 Injector

Precise nanoliter-volume injections with intuitive SMARTouch™ controller

NANOLITER2020 is the complete system. 300704 is the NANOLITER2020 Injector Head only, and the controller and the accessories are not included.

• Perform nanoliter volume injections down to 25nL using glass micropipettes
• Nanoliter Injector works on the principle of Positive Displacement. An internal micrometer step motor precisely moves the metal plunger which pushes the oil inside the micropipette, and the oil-layer pushes nanoliter volumes of aqueous sample out of the pipette tip.
• Fine control of plunger displacement along with proper sealing among gasket, glass micropipette, and oil ensures precision and accuracy
• NANOLITER2020 System includes the 300704 NANOLITER Injector, the MICRO2T SMARTouch™ Controller and many necessary accessories (See Details)

 

Benefits

• Minimizes loss of costly or scarce samples because injections are performed with mineral oil, back-filled, glass micropipettes, and the sample is front filled.
• Control up to two pumps simultaneously
• Easily mounted on a micromanipulator (e.g., M3301) or a stereotaxic frame (e.g. 505313) 
• Injections performed with mineral oil back-filled glass micropipettes, and the sample is front filled. This approach minimizes loss of any costly and scarce sample when a minute sample volume can be adequate. (See FAQ for details.)
• Precise control over injection volumes (in the nanoliter to microliter range) and injection rates
• Simple to use touchscreen interface and graphical representation of volume status on MICRO2T controller
• LED indicator offers a visual indication of connectivity
• Perform injections with a foot switch(optional)
• Injector is compatible with different OD glass micropipettes (in the range of 1.1-1.5 mm)

Applications

The NANOLITER2020 Injector is perfect for making nanoliter to microliter range injections into frogs (Xenopus oocytes), rats, mice, mosquitoes, shrimps, insects (e.g., Brown Planthopper), flies (e.g., Drosophila) and fish (Zebrafish) embryo using glass micropipettes.

 

Important Notes

• MICRO2T SMARTouch™ controller is required to operate the 300704 Injector (The same MICRO2T controller can be used with UMP3 pumps.)

• 300746 Spare Parts Kit required for use with the 300704 Injector

• Glass micropipettes are not included with the 300704. Use WPI glass micropipettes or use glass capillaries (504949 or 504950) and a puller to make micropipettes.

• Use a tweezer (501981) to scoop gaskets out of the NANOLITER2020 Injector Head

• 300704 Injector Head comes with one set of gaskets installed in the injector to use with 1.1-1.15 mm OD fire-polished glass

 

Options

Order code	Description
NANOLITER2020	Complete system (Injector and Controller)
300704	NANOLITER2020 Injector Head only

 

NANOLITER2020 Improvements

• Connects directly to our MICRO2T SMARTouch™ controller, which can also be used with WPI’s UMP3 pump
• The injector head has a LED indicator which lights up bright red in RUN mode and dim red when connected to the controller. The LED indicator shows proper communication between the pump and controller.
• 1.14 mm OD fire polished glass micropipette is recommended for use with front green gasket. Use different OD fire-polished glass micropipettes by switching the front gasket (Green gasket for 1.1-1.15 mm OD glass, Black for 1.3-1.35 mm, Red for 1.5 mm).
• Improved precision by in-depth plunger displacement validation
• System is easier to use or operate
• Injector has a chic silver design

Components

NANOLITER2020 System includes all the components listed below.

NOTE: The 300704 Injector may also be purchase separately. 300704 does not include the Controller or any of the required accessories listed in the table below.

Order code	Description	Quantity
300704	NANOLITER2020 Injector Head [One Set of Gasket (Front Green Gasket) to use with 1.1-1.15 mm Glass is installed]	1
Items listed below are already included with NANOLITER2020 System or can be purchased separately:
MICRO2T	SMARTouch Controller	1
501981	Tweezers to scoop out gaskets	1
504949	Glass Capillary (ID = 0.530 mm, OD 1.14 mm)	1 (pack of 300)
TIP10XV119	Glass Micropipettes (ID = 0.530 mm, OD 1.14 mm, Tip ID= 10 μm)	2
300746 Spare Parts Kit includes:
	14456 Allen Wrench 0.035 Hex Tool	1
	MF34G MicroFil 34 ga	1
	300733 O-Ring Kit for NANOLITER2020	5
	3563 1 cc Syringe	1
	300514 Replacement Piston for NANOLITER2020	2

Two sample glass micropipettes are included. Use WPI-made glass micropipettes or use a glass capillary (504949) (included with NANOLITER2020) and a puller to make your own micropipettes.

NANOLITER2010 vs NANOLITER2020

Type	NANOLITER 2010 Injector	NANOLITER 2020 Injector
Compatibility with 1.14 mm OD Fire-polished Glass Micropipettes	Yes	Yes
Compatibility with 1.3- or 1.5-mm OD Fire-polished Glass Micropipettes	No	Yes
Mount (500778) included to attach onto a micromanipulator or stereotaxic frame	Yes	No (different mounting bar included)
Compatible with Standard Controller	Yes	No
Compatible with MICRO2T and MICRO4 Controller	Yes	Yes
Adapter to connect to MICRO2T is Required	Yes	No
LED Indicator is Present	No	Yes
Foot Peddle Controllable	Yes	Yes

NANOLITER2020 Brochure

NANOLITER2020 Injector Quick Start Guide

NANOLITER2020 Instruction Manual

Troubleshooting Jammed NANOLITER2020

Video

Five reasons to try the NANOLITER2020 Microinjector

Using WPI's New NANOLITER2020 Injector

Setting Up Your Nanoliter 2020

Front Filling a Micropipette with the WPI Nanoliter2010 Injector

Nanoliter2010 Replace Gasket

 

Frequently Asked Questions (FAQ)

• What is the advantage of using oil back-filled glass micropipettes?

The term “back filling” refers to the process of filling the pipette from the large, non-pulled end.  “Front filling” is the term which is used to describe filling a micropipette through the small, pulled front end of the pipette. The glass micropipettes are first backfilled completely with mineral oil and secured to the NANOLITER Injector Head. Then, some mineral oil is dispensed through the tip. This creates the space and generates the pressure to front-fill samples through the tip. Front filling sample prevents the spillage or loss of costly or scarce sample involved with back-filling samples. When the sample volume is low, first back-filling glass micropipettes with oil and then front-filling the sample may be the only option.

 

• Can I use WPI’s old standard controller to run the 300704 Injector head?

WPI NANOLITER2020 Injector Head (300704) needs the MICRO2T controller (recommended). WPI’s MICRO4 controller may be used to control the pump. The 300704 Injector Head cannot be used with WPI’s old standard controller.

 

• Can I use same MICRO2T controller that I use with UMP3 pump? Do I need an additional adapter to use the 300704 pump with my existing MICRO2T controller?

Yes, the same MICRO2T controller can be used to control the UMP3 pump and the Nanoliter2010. No additional adapter is needed. 300704 connects directly to MICRO2T controller.

 

• Can I run the pump using a footswitch?

Yes, you can use the footswitch 13142. This is not included in the NANOLITER2020 system and is sold separately.

 

• What do I do when glass micropipettes come off after attaching or I am unable to front-fill a sample?

Odorless and colorless mineral oil is used for backfilling glass micropipettes. While the glass micropipette is installed and with gradual spillage over multiple uses, the gaskets become too slippery to hold glass micropipette in place. Use Kimwipes to soak up oil spillage inside the injector head, outside the glass micropipettes and to clean the gaskets. Wipes sprayed with 70% ethanol or isopropanol can be used for cleaning. If cleaning the oil spillage did not solve the issue, a new set of gaskets needs to be installed.

 

• I position the  O-rings  in the right order and orientation per your video but still don’t get an air tight seal .What can be done?  

If the rear seal is loose or mounted backwards then it can leak from air passage. This gasket needs to be tight on the plunger wire. If the pipette is pull tested, then it can be easily unseated from the middle gasket and leak by air infusion. The butt end of the glass needs to be smooth (and without any cracks in the glass) and firmly held down onto the middle seat while the collet is being screwed down. That plastic seat will deform a small amount and "mold" itself to the end of the glass. The fluids can also flow out on their own without any added back pressure even if there is a good seal, but that amount of mixing at a 10 um tip is very small. If either rear gasket allows for air flow, then it will leak.

NANOLITER2020/300704 Injector Specifications

Type	Description
NANOLITER 2020 Plunger Outer Diameter	482 µm
Plunger Movement for 100 nL Volume Dispense	550 µm ± 55 µm
Piston Movement per dispensed volume (nL)	5.5 µm/nL
Linear Travel Per Full Step	12.7 µm/step
Glass Use Capabilities	1.10-1.15 mm OD Fire-polished Glass with Green Front gasket 1.30-1.35 mm OD Fire-polished Glass with Black Front Gasket 1.5 mm OD Fire-polished Glass with Red Front Gasket
Recommended Glass	TIP10XV119 (Micropipette) 1.14 mm OD Fire-polished Glass Capillaries (504949 & 504950)
Recommended Minimum Injection Volume	25 nL with 1.14 OD Glass (TIP10XV119 and Green Front Gasket) 50 nL with 1.5 mm OD Glass (1.5 mm OD Fire-polished Glass and Red Front Gasket)
Maximum Injection Volume	4200 nL
Minimum Injection Volume	12.5 nL
Maximum Rate	644 nL/sec