A Quick Guide on the Common Uses for Lab Forceps
One of the most common surgical instruments in a laboratory is lab forceps or tweezers. Thumb forceps used in a lab come in various lengths, and the tips can be straight, curved, angled, or angled on the side. They can be made of stainless steel, German steel, Dumoxel, Dumastar, or titanium. They can have smooth tips, serrated tips, or teeth. They are even available with tungsten carbide inserts. Each style has its own purpose.
The Different Surgical Scissors for Every Application
In a life science laboratory, quality surgical scissors are indispensable for dissection, suturing, small animal surgeries, tissue preparation, and more. The variety of surgical scissors can be overwhelming. To help you choose which surgical scissors are right for your application, let’s look at some of our most popular ring handled surgical scissors and their intended uses.
EVOM™ Auto: Fix Software Switching Modes
If the EVOM™ Auto software switches from Resistance to TEER mode while you are selecting menu options, here's a quick tip:
Be careful when selecting Resistance mode, do not tap the background window and do not click on the side arrows.
Tapping the background window will switch you to TEER mode.
Instead, tap an option on the forefront square window to stay in Resistance mode.
Explore the Difference Between Tissue and Dressing Forceps
Surgical forceps or surgical tweezers are common surgical instruments, but there's a large variety of tweezers to choose from. In general forceps can be grouped as thumb forceps (frequently called surgical tweezers, gripping forceps, non-locking forceps or pinning forceps) or ring forceps (also called hemostats, hemostatic forceps and locking forceps).
APP NOTE: Common Applications of the EVOM™ Auto System
EVOM™ Auto is the latest generation of WPI’s automated transepithelial or transendothelial electrical resistance (TEER) measurement system. Using the same proven technology in the EVOM™ Manual and REMS, combined with a new multi-electrode array, software interface and control system, it delivers our fastest workflow solution while improving TEER measurement accuracy:
VIDEO: How to Schedule an Operation on the EVOM™ Auto
The EVOM™ Auto automates measurements of TEER in epithelial or endothelial monolayers cultured on high throughput screening 96-well plates utilizing our innovative EVOM™ technology, qualitatively measuring cell monolayer health and quantitatively measuring cell confluence by determining an increase or a plateau in tissue resistance.
The video below shows you how to schedule an operation on the EVOM™ Auto:
VIDEO: How to Stabilize the Electrode Array on the EVOM™ Auto
The EVOM™ Auto automates measurements of TEER in epithelial or endothelial monolayers cultured on high throughput screening 96-well plates utilizing our innovative EVOM technology, qualitatively measuring cell monolayer health and quantitatively measuring cell confluence by determining an increase or a plateau in tissue resistance.
The video below shows you how to stabilize the electrode array on the EVOM™ Auto:
VIDEO: Quick Reference for Sterilization of Surgical Instruments
After a good cleaning and disinfection, your surgical instruments may be sterilized using a variety of methods. The chart below shows two common methods, autoclaving and cold sterilization. Sterilization destroys all microbial life. Some chemical sterilants can be used as high level disinfectants (HLD) when used for shorter exposure periods.
VIDEO: How to Create a Plate File on the EVOM™ Auto
The EVOM™ Auto automates measurements of TEER in epithelial or endothelial monolayers cultured on high throughput screening 96-well plates utilizing our innovative EVOM™ technology, qualitatively measuring cell monolayer health and quantitatively measuring cell confluence by determining an increase or a plateau in tissue resistance.
VIDEO: How to Download and Delete a Data File on the EVOM™ Auto
The EVOM™ Auto automates measurements of TEER in epithelial or endothelial monolayers cultured on high throughput screening 96-well plates utilizing our innovative EVOM™ technology, qualitatively measuring cell monolayer health and quantitatively measuring cell confluence by determining an increase or a plateau in tissue resistance.
The video below shows you how to download and how to delete a data file EVOM™ Auto:
PRESS RELEASE: Introducing EVOM Auto for TEER in HTS
World Precision Instruments (WPI) is excited to launch the newest member to its EVOM™ family of products, the EVOM™ Auto. The new EVOM™ Auto enables rapid and reproducible measurements of Transepithelial Electrical Resistance (TEER),
a well-established and widely accepted method of evaluating epithelial and endothelial cells and tissues, in vitro. WPI’s EVOM™ Auto combines TEER technology with high throughput screening capabilities, resulting in the automated measurement of resistance in 96-well cell culture plates. This data gives scientists and drug discovery laboratories insights into tissue and monolayer barrier integrity, cell permeability, cell confluency, and can readout the critical physiology of barrier tissues such as the blood-brain barrier, the gastrointestinal tract, the lung, and the skin.
VIDEO: EVOM™ Auto, Introducing the Experiment Screen
The EVOM™ Auto automates measurements of TEER in epithelial or endothelial monolayers cultured on high throughput screening 96-well plates utilizing our innovative EVOM™ technology, qualitatively measuring cell monolayer health and quantitatively measuring cell confluence by determining an increase or a plateau in tissue resistance.
The video below shows details the features and functions of the Experiment window on the EVOM™ Auto:
VIDEO: Introducing the EVOM™ Auto Home Screen
The EVOM™ Auto automates measurements of TEER in epithelial or endothelial monolayers cultured on high throughput screening 96-well plates utilizing our innovative EVOM™ technology, qualitatively measuring cell monolayer health and quantitatively measuring cell confluence by determining an increase or a plateau in tissue resistance.
The video below shows you the features of the homepage on the EVOM™ Auto:
APP NOTE: TEER Technology Enables an Optimized in vitro Workflow for Drug Discovery
In vitro models have employed two common methods to quantify changes in endothelial barrier integrity: transepithelial/transendothelial electrical resistance (TEER) and tracer compound permeability.1 TEER is a non-invasive method that quantifies changes in electrical conductance to measure confluency and barrier integrity. Tracer compound permeability uses molecules of defined molecular weights to measure the size exclusion capacity of cell barriers (e.g., 4 kDa FITC-dextran or FD4).1 Using the EVOM™ Manual (EVOM-MT-03-01) with the EndOhm TEER electrode and cell culture permeable supports, this application note describes how to non-invasively evaluate endothelial barrier integrity after cytokine treatment and provides a method to identify vasoactive compounds that have the potential to induce vascular injury. Tracer compound permeability studies are combined with TEER evaluation to elucidate treatment-induced impacts on both intercellular junctions and paracellular transport (Fig. 1).
APP NOTE: A Comparative Time Study of the EVOM™ Auto versus the REMS
World Precision Instruments' (WPI) legacy Robotic Epithelial Measurement System (REMS) and the new advanced, upgraded version of REMS, EVOM™ Auto, are high-throughput, automated transepithelial/transendothelial electrical resistance (TEER) measurement systems. These robotic systems, combined with EVOM™ technology, expedite TEER measurements.
APP NOTE: Observation of Mitosis using Celloger® Mini Plus
In the process of ‘cell cycle’, cells grow and divide into two genetically identical daughter cells. It is regulated by a complex signaling pathway which keeps cell homeostasis by regulating cell division and DNA duplication1. On the other hand, because cancer cells grow and divide indefinitely out of cell cycle control, anti-mitotic drugs are used to suppress abnormal proliferation of cancer cells2. In particular, Nocodazole is known to be a representative anti-mitotic drug for cancer treatment, and it has the characteristics of disturbing microtubule dynamics during cytoplasmic and nuclear division3,4.
VIDEO: How to Set up the EVOM Auto TEER Measurement System
Have you got the new EVOM Auto TEER Measurement System for 96-well HTS plates? This short videos shows how easy it is to setup.
APP NOTE: Analysis of Nocodazole-Induced Cytotoxicity Using Celloger® Mini Plus
Cytotoxicity refers to the degree of damage to cells. caused by chemical substances or physical factors. Measuring it through cytotoxicity assay is essential for drug development and biological research. Cells undergo complex signaling pathways that causes various cell death processes such as apoptosis, necrosis, and necroptosis. However, most cytotoxicity assays are measured at the endpoint that makes it difficult to study the dynamic response of cells to drugs.
APP NOTE: Using Celloger® Mini Plus to Observe Morphological Changes and Phagocytic Activity in Macrophage Cell Line
As white blood cells responsible for immune function are suspension cells that travel along blood vessels, immunology studies often use various suspension cell lines originating from white blood cells. Dealing with suspension cells, unlike adherent cells, slight movement of a plate when locating it on the microscope causes the cells to float. Aside from the problems caused by temperature and CO2 instability, it is in fact not possible to use a traditional microscope to monitor cells in real time. Therefore, in order to stably monitor suspension cells, a live cell imaging device such as Celloger® Mini Plus that operates inside an incubator is essential1. In addition, with Celloger® Mini Plus, the camera inside the system moves to capture the images of cells in multiple positions to keep the cell sample in a steady state instead of having a movable stage with a plate on it. When the suspension cells were monitored both by Celloger® Mini Plus and microscope, imaging with Celloger® Mini Plus was more stable compared to using a microscope in which several cells were out of focus (Figure 1).
NEWS: FDA Modernization Act Became Law
The US FDA Modernization Act 2.0 replaces the 1938 Federal Food, Drug and Cosmetic Act which required animal testing prior to human trials. The new law, sponsored in the Senate by Rand Paul and co-sponsored by 11 other bi-partisan senators, eliminates that requirement. Representatives Vern Buchanan and Elaine Luria introduced the companion bill in the House, with over 105 bi-partisan cosponsors. President Biden signed the bill into law in December 2022.
PRESS RELEASE: WPI & MatTek Jointly Announce New Distribution Agreement
WPI is very excited to announce our newest product launch and collaboration with MatTek Life Sciences. The companies will offer both MatTek PermaCell Cell Culture Insert Plates and compatible customized World Precision Instruments’ STX HTS EVOM™ Electrode for TEER to their respective customers, available immediately to the life sciences market.
VIDEO: Using the Adaptive Mode on the ATC2000
The ATC2000 is a low noise heating system for maintaining animal body temperature during expirimental procedures. Here we show you how to use the adaptive mode on the ATC2000 system.
VIDEO: Unboxing the EVOM™ Manual TEER meter
EVOM™ Manual is WPI's newest instrument to measure Trans Epithelial Electrical Resistance (TEER). Let's look at what comes with the system and how easy it is to setup.
Common Applications for EVOM Manual
WPI's EVOM system is popular in the research community, both in academia and in industry, and it is commonly used for the evaluation of mammalian cellular health by measuring transepithelial/transendothelial electrical resistance (TEER or TER) of cellular layers.
EVOM™ Manual is powered by the same EVOM™ technology as older EVOM models (EVOMX, EVOM, EVOM2 and EVOM3). It has advanced features for performing experiments more easily. With the new touchscreen display you can now store data as Microsoft® Excel files on a USB flash drive. Just remove the flash drive with all your recorded data from the EVOM™ Manual and plug it into a computer to access and plot your data. It is as simple as it sounds.