VIDEO: How to Pull Glass using the PUL-1000 Micropipette Puller

The PUL-1000 is a microprocessor controlled, four-stage, horizontal puller for making glass micropipettes or microelectrodes. Here is how to run a program that will pull two identical microelectrodes.

VIDEO: Getting Started with your PUL-1000 Micropipette Puller

The PUL-1000 is a microprocessor controlled, four-stage, horizontal puller for making glass micropipettes or microelectrodes. Here is a quick overview to get you started qui

How to Load Capillary Glass in a PUL-1000 Micropipette Puller

The PUL-1000 is a microprocessor controlled, four-stage, horizontal puller for making glass micropipettes or microelectrodes. Here we will see how to load a piece of glass into the carriage.

VIDEO: How to Run a Glass Softening Test on the PUL-1000 Puller

The PUL-1000 is a microprocessor controlled, four-stage, horizontal puller for making glass micropipettes or microelectrodes. We will show you how to run a glass softening test.

VIDEO: Researcher's Review of the EVOM3

The EVOM is a special kind of voltmeter for measuring electrical properties across a layer of cells in culture or a biological membrane. It is the most commonly used commercial system for measuring across a layer of cultured cells. We are measuring TEER (TER) - Transepithelial Electrical Resistance. The EVOM3 allows us to zoom in and measure either resistance or voltage across these membranes or cells in culture.

VIDEO: Researcher Compares EVOM3 with an Ussing Chamber

An Ussing Chamber is used when performing barrier studies across an excised epithelial tissue. Similar measurements can be made in well plates using an EVOM series TEER Measurement Meter, which is used for epithelial tissue that is grown to confluence in a cell culture well. While EVOM measurements are only qualitative, your throughput is much greater with an EVOM3. The EVOMs are portable and much more economical than an Ussing Chamber. Here Ben Dubansky, PhD explains the differences between EVOMs and Ussing chambers.

Get Familiar with Your New PUL-1000 Micropipette Puller

Let’s look at the PUL-1000, a microprocessor controlled, four-stage, horizontal puller for making glass micropipettes or microelectrodes. Here we will examine the design of the unit.

How to Read a Vernier Scale

Vernier scales can be used on microscopesstereotaxic frames and micromanipulators. The vernier scale was invented by French mathematician Pierre Vernier in 1631 as an upgrade on Pedro Nunes' measurement system for precision astrolobes. With a main scale and a sliding secondary scale, a vernier is used for making precise measurements. 

VIDEO: Disposable Scalpels – Perfect for Researchers, Students and Crafters

WPI’s disposable scalpels come in eight different styles and can be used for a wide variety of applications. Here you can see what makes WPI inexpensive, disposable scalpels such a popular tool.

VIDEO: How to Choose a Scalpel Blade for Your Application

WPI’s disposable scalpels come in many styles and are ideal for students, researchers, and crafters. Here we will describe some of the uses for these popular blades.

VIDEO: How to Setup the NanoFil System for Injections

NanoFil™ is a unique low volume gas tight syringe developed for improved microinjection in mice and other small animals. Here we show you several ways in which the NanoFil can be used for making injections. You can install the syringe on WPI’s UMP3 UltraMicroPump, using SilFlex tubing and a holder, so you can accurately inject by hand. You can install it in a stereotaxic frame. You can also directly inject by hand.

VIDEO: How to Install the NanoFil Syringe Needles

NanoFil™ is a unique low volume syringe developed for improved microinjection in mice and other small animals. Here we'll show you how to install your needle in a way that prevents damage to the gasket and keeps your needles straight.

VIDEO: How to Install NanoFil Syringe on the UMP3

NanoFil™ is a unique low volume syringe developed for improved microinjection in mice and other small animals. Using a UMP3 UltraMicroPump combined with the syringe allows you to make repeatable injections in the nanoliter range. Here we'll show you how to install a NanoFil Syringe on the UMP3 UltraMicroPump.

VIDEO: How to Choose the Correct NanoFil Tip Size for Microinjections

NanoFil™ is a unique low volume syringe developed for improved microinjection in mice and other small animals. During our field trials, the 35-gauge needle was scientists’ most popular and preferred NanoFil tip. The combination of its strength, length, durability and clogging resistance creates a solid balance.

VIDEO: How to Use NanoFil Syringe for RPE and IO Injections

NanoFil™ is a unique low volume gas tight syringe developed for improved microinjection in mice and other small animals. RPE and IO kits are specially designed for “hands-free” and oil-free Gas Tight Injection System. Originally developed for retinal pigment epithelium and intraocular injection, they can also be used for other applications.

VIDEO: Why Buy a PUL-1000 Research Puller for Making Micropipettes?

PUL-1000 is a microprocessor controlled, four-stage, horizontal puller for making glass micropipettes or microelectrodes used in intracellular recording, patch clamp, microperfusion and microinjection. The PUL-1000 is capable of producing a vast array of pipette shapes, such as a long graduated taper, a short graduated taper and a short taper bee stingers. Here's a quick introduction the PUL-1000 research puller.

VIDEO: NanoFil Needles Cause Less Tissue Damage

NanoFil™ is a unique low volume syringe developed for improved microinjection in mice and other small animals. NanoFil needles are specially designed with a small outside diameter and spear point for shallow penetration and to cause the least amount of tissue damage. 

VIDEO: NanoFil Microliter Syringes for Accurate Microinjections

NanoFil™ is a unique low volume syringe developed for improved microinjection in mice and other small animals. It comes in both 10 and 100 microliter volumes and can be paired with a UMP3 UltraMicroPump for making accurate microliter injections. NanoFil stainless steel needles range in size from 33 to 36 gauge.

How to Mount an Electrode Holder on Your Micromanipulator

The M3301 is a popular micromanipulator used for laboratory research. Here we will show you how to mount a microelectrode holder on the M3301 Micromanipulator. The procedure is very similar for most manual micromanipulators.

Five Factors That Affect the Pulling of Glass Micropipettes

Pulling micropipettes or microelectrodes is a science and an art, requiring some finesse. Here we discuss the five major factors which can affect the shape of a pulled glass micropipette or microelectrode.

Magnetic Stands for Micromanipulators Give You an Extra Hand

Magnetic stands act as an invaluable extra hand around the laboratory to hold an electrode, digital dials or tools. The base houses a strong magnet which can be activated by turning the switch. Position the magnetic stand on a weighted steel base plate or metal desk and rotate the dial counter-clockwise to engage the magnet. We offer multiple styles of magnetic stands, but these videos show a couple options.

Affordable New Injector: Setup the PV850 System

The PV850 Injector is designed to simplify intracellular injection and a variety of other microinjection tasks. The PV850 uses regulated air pressure for injecting cells with fluid. Injected volumes range from picoliters to nanoliters. The port supplies positive pressure for high-pressure ejection maximum of 87 PSI.

Affordable New Injector: Configure the PV850 Software

The PV850 Injector is designed to simplify intracellular injection and a variety of other microinjection tasks. The PV850 uses regulated air pressure for injecting cells with fluid. Injected volumes range from picoliters to nanoliters. The port supplies positive pressure for high-pressure ejection maximum of 8 7PSI.

VIDEO: Setting up the WPI PicoPump

Designed to simplify intracellular injection and a variety of other micro­in­jec­tion tasks, WPI’s PicoPumps (PV830 and PV820) use precisely regulated pres­sures for se­cur­ing cells and injecting them with fluid. Injected volumes range from picoliters to nanoliters. Separate ports supply positive and negative pressure—pos­i­tive pressure for high-pressure ejection, and suction for supporting the cell or for filling the pipette from the tip. In this quick series, you can see how to setup a PicoPump.