World Precision Instruments is the leading manufacture of benchtop TEER instrumentation including the original equipment manufacturer of Millipore Millicell® ERS-2 (Part number MERS00002) and Millipore Millicell® ERS (Part number MERS00001).

WPI has manufactured TEER instrumentation, including the EVOM2, EVOM3, EVOM™ Manual, and Millicell® ERS-2 (a WPI OEM product), for over three decades. Millipore’s Millicell® ERS 3.0 recently became available and is NOT manufactured by WPI, nor validated or certified to be accurate for TEER measurement. WPI manufactured and validated product, like EVOM™ Manual, the proven gold standard for TEER measurement, ensure the accuracy and reproducibility of your work. Accept no substitutes.

Transepithelial/Endothelial Electrical Resistance (TEER) qualitatively measures cellular monolayer health and quantitatively evaluates cellular confluence by measuring the electrical resistance across a cell monolayer. It is commonly used to assess the integrity and permeability of cellular barriers, such as epithelial and endothelial cell layers in cell culture plates. Organ-on-Chip platforms are an emerging market for TEER applications. Let’s look at what TEER is and how it is used.

The EVOM™ Auto automates measurements of TEER in epithelial or endothelial monolayers cultured on 24-well or 96-well high throughput screening (HTS) plates utilizing our innovative EVOM technology, qualitatively measuring cell monolayer health and quantitatively measuring cellular confluence by reaching an increase or a plateau in tissue resistance. The autosampler has a 3-well rinse station for cleaning and stabilizing the electrodes. Now, sterile disposable rinse station inserts are available for your convenience. They fit over the 3-well base rinse station. You can simply throw out the disposable inserts with the solutions when you are changing plates. This short videos shows how easy they are to use.

Transepithelial Electrical Resistance (TEER) measurements play a vital role in assessing barrier function, tissue integrity, and cell-cell interactions in Organ-on-Chip (OoC) platforms. Measuring TEER by applying a small AC current across a monolayer of cells and measuring the resistance to that current, which provides valuable insights into the physiological relevance and functionality of in vitro models, enabling researchers to study organ-specific responses, disease mechanisms, and drug effects while cells are growing in a controlled microenvironment. This article will explore the applications of TEER in OoC set-ups, critical aspects to TEER measurements in microphysiological systems, and challenges and limitations that must be overcome to fully realize the potential of this technology for OoC platforms.

Cell therapies have revolutionized the field of regenerative medicine, offering promising treatments for a variety of medical conditions. Maintaining the safety, efficacy, and consistency of cell-based therapies is paramount for their successful application in clinical settings. Quality control measures play a vital role in evaluating the quality of these therapies, from development to administration. This article explores the current approaches to quality control in cell therapies, including the utilization of functional measurement techniques such as transepithelial electrical resistance (TEER), to ensure the quality and safety of these promising treatments.

Transepithelial electrical resistance (TEER) has emerged as a powerful tool in ophthalmology research and has become a standard method for study of retinal pigment epithelial (RPE) cell biology and the development of these cells as a therapeutic for retinal diseases such as Age-Related Macular Degeneration (AMD). The application of TEER in assessing the barrier function and integrity of RPE cell layers has significantly advanced our understanding of retinal diseases and paved the way for innovative therapeutic interventions including some of the most promising cell therapies to date. This article explores the use of TEER in ophthalmology research, with a focus on studies utilizing RPE cells for both research and clinical applications.

EVOM™ Auto, with both 24 and 96 HTS multiwell plate capability, is a high throughput screening (HTS) platform offering fast, noninvasive sample scanning by comparing electrical resistance measurements (transepithelial/transendothelial electrical resistance: TEER). TEER measurement experiments are simple to set up and less time consuming than more complex molecular studies. TEER measurement can be used as the primary scanning method to identify any physiological changes that can then be further evaluated by other methods. EVOM™ Auto can capture TEER measurements in 24 and 96 HTS multiwell plates from Corning, Millipore, or MatTek. The sample preparation time in these HTS plates is efficient, allowing for fast, multi-channel pipetting options.

The EVOM™ Auto autosampler ships with a red electrode array lock in place to prevent the electrode array connection port from moving during transit. Here you can see how to easily remove it without damaging the lock or the autosampler.

Choosing a dependable lab equipment supplier is crucial for ensuring the quality, reliability, and timely delivery of the equipment you require.  When your research is on the line, you need a company you trust, a supplier with a proven track record and a service team to back you up. Here are a few important considerations when selecting a lab equipment supplier.

The EVOM™ Manual requires an electrode for use. Choose an electrode suitable for your application. Here's a matrix to help you choose an appropriate EVOM electrode.

The EVOM™ Auto automates measurements of TEER in epithelial or endothelial monolayers cultured on high throughput screening 96-well plates utilizing our innovative EVOM™ technology, qualitatively measuring cell monolayer health and quantitatively measuring cell confluence by determining an increase or a plateau in tissue resistance.

The video below shows you how to schedule an operation on the EVOM™ Auto:

The EVOM™ Auto automates measurements of TEER in epithelial or endothelial monolayers cultured on high throughput screening 96-well plates utilizing our innovative EVOM technology, qualitatively measuring cell monolayer health and quantitatively measuring cell confluence by determining an increase or a plateau in tissue resistance.

The video below shows you how to stabilize the electrode array on the EVOM™ Auto:

The EVOM™ Auto automates measurements of TEER in epithelial or endothelial monolayers cultured on high throughput screening 96-well plates utilizing our innovative EVOM™ technology, qualitatively measuring cell monolayer health and quantitatively measuring cell confluence by determining an increase or a plateau in tissue resistance.

The video below shows you how to download and how to delete a data file EVOM™ Auto:

The EVOM™ Auto automates measurements of TEER in epithelial or endothelial monolayers cultured on high throughput screening 96-well plates utilizing our innovative EVOM™ technology, qualitatively measuring cell monolayer health and quantitatively measuring cell confluence by determining an increase or a plateau in tissue resistance.

The video below shows details the features and functions of the Experiment window on the EVOM™ Auto:

The EVOM™ Auto automates measurements of TEER in epithelial or endothelial monolayers cultured on high throughput screening 96-well plates utilizing our innovative EVOM™ technology, qualitatively measuring cell monolayer health and quantitatively measuring cell confluence by determining an increase or a plateau in tissue resistance.

The video below shows you the features of the homepage on the EVOM™ Auto:

In vitro models have employed two common methods to quantify changes in endothelial barrier integrity: transepithelial/transendothelial electrical resistance (TEER) and tracer compound permeability.1  TEER is a non-invasive method that quantifies changes in electrical conductance to measure confluency and barrier integrity. Tracer compound permeability uses molecules of defined molecular weights to measure the size exclusion capacity of cell barriers (e.g., 4 kDa FITC-dextran or FD4).1  Using the EVOM™ Manual (EVOM-MT-03-01) with the EndOhm TEER electrode and cell culture permeable supports, this application note describes how to non-invasively evaluate endothelial barrier integrity after cytokine treatment and provides a method to identify vasoactive compounds that have the potential to induce vascular injury.  Tracer compound permeability studies are combined with TEER evaluation to elucidate treatment-induced impacts on both intercellular junctions and paracellular transport (Fig. 1).

World Precision Instruments' (WPI) legacy Robotic Epithelial Measurement System (REMS) and the new advanced, upgraded version of REMS, EVOM™ Auto, are high-throughput, automated transepithelial/transendothelial electrical resistance (TEER) measurement systems. These robotic systems, combined with EVOM™ technology, expedite TEER measurements.

Have you got the new EVOM Auto TEER Measurement System for 96-well HTS plates? This short videos shows how easy it is to setup.

EVOM™ Manual is WPI's newest instrument to measure Trans Epithelial Electrical Resistance (TEER). Let's look at what comes with the system and how easy it is to setup.

WPI's EVOM system is popular in the research community, both in academia and in industry, and it is commonly used for the evaluation of mammalian cellular health by measuring transepithelial/transendothelial electrical resistance (TEER or TER) of cellular layers. 

EVOM™ Manual is powered by the same EVOM™ technology as older EVOM models (EVOMX, EVOM, EVOM2 and EVOM3). It has advanced features for performing experiments more easily. With the new touchscreen display you can now store data as Microsoft® Excel files on a USB flash drive. Just remove the flash drive with all your recorded data from the EVOM™ Manual and plug it into a computer to access and plot your data. It is as simple as it sounds.

WPI’s EVOM™ Manual is the gold standard for delivering stable and repeatable Trans Epithelial Electrical Resistance (TEER) measurements. The EVOM™ Manual qualitatively measures cell monolayer health and quantitatively measures cell confluence by determining an increase or a plateau in tissue resistance detected using our innovative EVOM™ technology. The main display window has lots of information. Here’s a quick overview. You can download the infographic for a handy reference.