VIDEO: How to Take Voltage Measurements with the EVOM™ Manual
EVOM™ Manual is WPI’s newest instrument to measure Trans Epithelial Electrical Resistance (TEER). Let’s look at how to set it up and take voltage measurements.
VIDEO: 9 Reasons to Trust the EVOM™ Manual TEER Measurement Meter
WPI’s EVOM™ technology is the gold standard for delivering stable and repeatable Trans Epithelial Electrical Resistance (TEER) measurements. Here are nine reasons to trust the new EVOM Manual TEER Meter for qualitatively measuring cell monolayer health and quantitatively measuring cell confluence.
VIDEO: How to Take Resistance Measurements with EVOM™ Manual
EVOM™ Manual is WPI’s newest instrument to measure Trans Epithelial Electrical Resistance (TEER). Let’s look at how to set it up and take resistance measurements.
VIDEO: How to Replace the STX4 Electrode Blades
The new STX4 electrode works with the EVOM3, and the blades are replaceable. EVOM3 is used for making TEER (TER) Measurements in cell culture plates. Here's a quick tutorial to show you how to replace the blades.
Organ-on-Chip, TEER, and New Drug Development
The process of drug discovery and development is extremely slow and expensive. Regulatory authorities, such as US FDA need animal models to test absorption, distribution, metabolism, and excretion (ADME) which estimates safety and effectiveness of a drug.
VIDEO: 7 Reasons to Love the New EVOM3 for TEER Measurement
The NEW EVOM3 Epithelial Volt/Ohm Meter delivers improved workflow efficiency, increased stability and more repeatable measurements than traditional Trans Epithelial Electrical Resistance (TEER) meters. Here are seven things you will love about the new EVOM3.
How to Set the Gap on your WPI EndOhm Chambers
WPI EndOhm chambers are used with WPI's EVOM2 meter for making TEER (transepithelial electrical resistance) measurements. Each EndOhm chamber comes with a "spacer" disk for calibrating the gap between the two chamber electrodes. A consistent gap ensures reliable measurements. Here Subhra shows how to calibrate your chambers.
Resurfacing Electrodes in a WPI EndOhm Chamber
WPI EndOhm chambers are used with WPI's EVOM2 meter for making TEER (transepithelial electrical resistance) measurements. After heavy use your background resistance measurement may start to rise. Your electrodes may need to be resurfaced to remove buildup of proteins, sugars and biological materials. Here Subhra demonstrates how to clean and resurface your EndOhm chambers.
Choosing an EndOhm Chamber Compatible with your Cell Culture Inserts
WPI's EndOhm chambers are used with WPI's EVOM2 meter to measure TEER (transepithelial electrical resistance) in cell culture inserts. Three chamber sizes are available for 6-well plates, 12-well plates and 24-well plates. Here Subhra demonstrates how to choose an EndOhm chamber to match the cell culture inserts you are using.
Endohm Maintenance for Long Life
The EndOhm series of chambers, when used with WPI’s EVOM2 resistance meter or the Millicell ERS, are designed to provide reproducible and accurate resistance measurements of endothelial tissue in culture cups. Resistance values obtained with the EndOhm are consistent with those obtained using a well-designed Ussing Chamber.
Monitor the Growth of Epithelial Cells in Vitro Manually
Are you ready to perform experiments involving a cultured cell monolayer? If your monolayer has not reached confluence, you may have holes or gaps in the monolayer that would prevent collection of valid experimental data. When confluence is reached, the electrical resistance across the monolayer peaks and then plateaus. The EVOM2 meter was designed specifically for this application.
The EVOM2 is the ideal meter for use when you are making manual TEER measurements on small batches of cell cultures and do not require a high throughput system.
FAQs about TEER Measurement
Here are some frequently asked questions (FAQ) about TEER measurement using an EVOM2.
TEER Measurement in Individual Endothelial Cell Cultures Cups
WPI's EndOhm chambers were designed for making TEER measurement of endothelial cell cultures in individual cups. The EndOhm chambers feature:
Compatible with EVOM²
Improved accuracy of 1-2Ω
Accommodates 6mm, 12mm, 24mm cups and Costar Snapwell cup
Sterilized with EtO, alcohol or a bactericide
TEER Research: WPI's Manual and Robotic Systems
Transepithelial Electrical Resistance TEER measurements is the most convenient, reliable and non-destructive method for evaluating and monitoring the growth of epithelial tissue cultures in vitro. The confluence of the cellular monolayer is quickly determined by a sharp increase in TEER. TEER measurement technology, which was first introduced by WPI in the mid-1980's, has since been perfected and expanded to include a range of TEER related manual and automatic instrumentation, including:
EVOM² - Manual TEER measurement of epithelial cells in 24- and 96-well plates
REMS AutoSampler - Automated system for High Throughput Screening (HTS)
EVOM2 Remix
EVOM was the first instrument designed to perform routine Trans Epithelial Electrical Resistance (TEER) measurement in tissue culture research. EVOM2 is the next generation, redesigned for greater usability.
EVOM or Volt Meter: Defining the Difference
How To Test Your EVOM2 Meter
The World Precision Instruments (WPI) EVOM2 is used for making trans epithelial electrical resistance (or TEER) measurements. We recommend that you test your EVOM2 before you use it for the first time and then periodically after that. If there is a concern that the meter or the electrode is not functioning properly, test the meter first. This video shows how to test the meter.
EVOM2-How to Equilibrate Your STX2
The World Precision Instruments (WPI) EVOM2 is used for making trans epithelial electrical resistance (or TEER) measurements. Two silver/silver chloride pellets are used for the electrodes. The chloride ion tends to migrate, which is a reversible reaction. But, because of this chloride ion swapping, the electrodes need to be polarized before each use. Before using the meter for making voltage measurements, equilibrate the electrodes. This video shows you how.