Gas-Tight Microinjection System

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Regular price $140
Sale price $140 Regular price

NANOFIL

Regular price From $101 - $140
Sale price From $101 - $140 Regular price

Volume (uL)

Prices valid in USA, Canada, and PR only.

The NanoFil™ Gas-Tight Injection System boasts zero dead volume, an the variability is eliminated with the NanoFil™  design: our interchangable needles advance directly into the syringe barrel, creating a 1:1 connection with the plunger— a truly gas-tight system built for low-volume sample control. Our cross-compatible NanoFil™ needles are offered in sizes from 33-36g, blunt or beveled. 

* Returns Policy : Non-returnable/Non-refundable

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Curious how NanoFil™ supports precision microinjection?

Visit the Gas-Tight Injection System page to learn about its gas-tight design, needle versatility, etc.

ACCESSORIES




Details

Options

Order code Size Includes
NANOFIL 10 μL
  • one 26 gauge beveled needle 
  • 2 - MF28G MicroFil, 1 - 1CC syringe
NANOFIL-100 100 µL
  • One 26 gauge beveled needle 

 

Features & Applications

Nanofil bevel tip

  • Biologically compatible injection syringe
  • Compatible with WPI's UMP3 microinjector for precise, targeted injections
  • Animal research, viral targeting (e.g. AAV construct, DREADDS):
    • Retinal Pigment Epithelial (RPE)/ Intra Ocular (IO)
    • Brain
    • Muscle
    • Spine
  • Capillary electrophoresis

 

Try our quartz 34G flexible tubing for precision capillary backfilling (SKU #NFQ34-5). Particularly useful for low-volume sample transfer into long taper glass pipettes. Compatible with any NanoFilTM syringe!

  

neuroscienceophthalmologyviral injectionsCapillary Electrophoresis

   

Special Application Kits

ultramicropump with rpe kit

More Information

How to select the correct tip for your application.

See why our system is the choice for low-volume applications, and why other designs are not dependable: Tired of Missing the Mark? 

*Please see page 11 and 12 of the manual for NanoFilTM syringe and needle cleaning instructions.

 

 

Note: This product is non-returnable/non-refundable. For details, please refer to the WPI Terms & Conditions.

*After repeated use, it is normal that your plunger may incur wear/tear to either the PTFE tip and/or its overall straightness. The syringe must be replaced as plungers are specially made to each syringe. 

 

References

Guo, Q., Gobbo, D., Zhao, N., Zhang, H., Awuku, N.O., Liu, Q., Fang, L.P., Gampfer, T.M., Meyer, M.R., Zhao, R., Bai, X., Bian, S., Scheller, A., Kirchhoff, F., & Huang, W. (2024). Adenosine triggers early astrocyte reactivity that provokes microglial responses and drives the pathogenesis of sepsis-associated encephalopathy in mice. Nature Communications, 15; 6340. https://doi.org/10.1038/s41467-024-50466-y

Luo, S., Jiang, H., Li, Q., Qin, Y., Yang, S., Li, J., Xu, L., Gou, Y., Zhang, Y., Liu, F., Ke, X., Zheng, Q., & Sun, X. (2024). An adeno-associated virus variant enabling efficient ocular-directed gene delivery across species. Nature communications, 15(1), 3780. https://doi.org/10.1038/s41467-024-48221-4

Nam, J., Richie, C.T., Harvey, B.K., & Voutilainen, M.H. (2024). Delivery of CDNF byAAV‑mediated gene transfer protects dopamine neurons and regulates ER stress and inflammation in an acute MPTP mouse model of Parkinson’s disease. Communications Biology, 7; 966. https://doi.org/10.1038/s42003-024-06658-9

Wietek, J., Nozownik, A., Pulin, M., Saraf-Sinik, I., Matosevich, N., Gowrishankar, R., Gat, A., Malan, D., Brown, B.J., Dine, J., Imambocus, B.N., Levy, R., Sauter, K., Litvin, A., Regev, N., Subramaniam, S., Abrera, K., Summarli, D., Goren, E.M., Mizrachi, G., Bitton, E., Benjamin, A., Copits, B.A., Sasse, P., Rost, B.R., Schmitz, D., Bruchas, M.R., Soba, P., Oren-Suissa, M., Nir, Y., Wiegert, J.S., & Yizhar, O. (2024). A bistable inhibitory optoGPCR for multiplexed optogenetic control of neural circuits. Nature Methods, 21; pg. 1275–1287. https://doi.org/10.1038/s41592-024-02285-8

Zhang, C., Dulinskas, R., Ineichen, C., Greter, A., Sigrist, H., Li, Y., Alanis-Lobato, G., Hengerer, B., & Pryce, C.R. (2024). Chronic stress deficits in reward behaviour co-occur with low nucleus accumbens dopamine activity during reward anticipation specifically. Communications Biology, 7; 966. https://doi.org/10.1038/s42003-024-06658-9

Resources

Documents

NanoFil™ Gas-Tight Injection System Manual

NanoFil™ Gas-Tight Injection System Spec Sheet

Tired of Missing the Mark? 

Selecting Your NanoFil™ Needle Tip

Video

 

How to Prime Your NanoFil Gas-Tight Syringe System


 

Unboxing Your NanoFil & Installing a Needle


 

In the video below, you can see how to front fill an Nanofil syringe. 


  

How to Choose the Correct NanoFil Tip Size for Microinjections

 

 

How to Use the NanoFil Syringe for Retinal Pigment Epithelial and Intraocular Injections

 

 

How to Install a NanoFil Syringe on an UltraMicroPump

 

 

How to Setup the NanoFil System for Making Injections

 

 

NanoFil Microliter Syringes for Accurate Microinjections

 

 

NanoFil Needle Cause Less Tissue Trauma By Design

 

Specifications

Nanofil schematic

Tip Order Number Tip O.D. (µm) Tip I.D. (µm) Tip Length (mm) Total Length (mm) Shank O.D.  (µm) Bevel Length (µm) Tip Material
NF33BV 210 115 10 40 460 ≈348 Stainless Steel
NF34BV 185 85 5 35 460 ≈290 Stainless Steel
NF35BV 135 55 5 35 460 ≈204 Stainless Steel
NF36BV

120

35 3 33 460 ≈156 Stainless Steel
NFQ34-5 160 100 55 75 460   Quartz
NF33BL 210 115 10 40 460 ≈0 Stainless Steel
NF34BL 185 85 5 35 460 ≈0 Stainless Steel
NF35BL 135 55 5 35 460 ≈0 Stainless Steel
NF36BL 120 35 3 33 460 ≈0 Stainless Steel
Silflex   100   35      
NF26BV 460 140   40 460   Stainless Steel

 

NANOFIL™ Syringe Specification Table

Syringe Order Code Volume Syringe Type Syringe Body Autoclavable/Gas Sterilizable Plunger Stroke Length Plunger Cap Dimensions

Syringe Barrel

I.D. / O.D.*

Qty MICRO4 / MICRO2T Syringe Type Selection
NANOFIL

10µL

Gastight

Borosilicate glass, Stainless Steel, PTFE

Yes ✓

10°C/50°F to 80°C/176°F, 1000psig max pressure

60mm

O.D.*: 7.90mm (0.311in)

Depth: 2.80mm (0.110in)

0.46mm (0.018in)           /  6.40mm (0.252in)

1 EA

L  (MICRO4)

5 (MICRO2T)

NANOFIL-100

100µL

0.46mm (0.018in)           /  6.40mm (0.252in)

1 EA

*I.D. = Inner diameter, O.D. = Outer diameter


Gas-Tight Microinjection System

$140.00

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