Micro Nitric Oxide Sensors

7 and 30 µm sensors with exceptional performance – ideal for tissues and microvessels

Don't forget the SNAP!

• Excellent selectivity to NO
• Rapid response time
• Highly sensitive
• For use with Apollo1000, Apollo4000, TBR4100 and TBR1025
• Requires cable 91580 (sold separate)
• Package of 3

Options

Order code	Length	Tip Diameter	Shape
ISO-NOP007	2 mm	7 µm	Straight
ISO-NOPF3005	0.5 mm	30 µm	Straight
ISO-NOPF3020	2 mm	30 µm	Straight

 

 Benefits

• Linear response over a large dynamic concentration range of NO
• Dry electrodes require no sleeves or filling solution

Applications

• NO measurement in microvessels

The ISO-NOP007 has a tip diameter of just 7 microns and a length of 2mm. The response is linear over a wide dynamic concentration range of NO. The design is based on a single carbon fiber coated with WPI's NO-selective membrane. A detection limit of approximately 1 nM NO makes this electrode ideal for use in tissues and microvessels.

The ISO-NOP30 has a tip diameter of 30 microns and is available in two different tip lengths ISO-NOP3020 has tip length of 2 mm, ISO-NO3005 has tip length of 0.5 mm). The response is linear over a wide dynamic concentration range of NO. The design is based on a single carbon fiber coated with WPI's NO-selective membrane. A detection limit of approximately 1 nM NO makes these electrodes ideal for use in tissues and microvessels.

The response of a 7μm NO sensor (ISO-NOP007) to successive additions of NO (100nM). Inset shows the linearity of the resulting calibration plot.

Simultaneous measurement of force (top trace) and changes of NO concentration (lower trace) in (A) the rat superior mesenteric artery relaxed with ACh and (B) a small human artery relaxed with ACh and SNAP. In this artery oxyhaemoglobin (oxyHb) partly reversed the increase in NO concentration with only a small change in force. [U. Simonsen, et al., J. Physiol., 1999, 516: 271-282.]

NO Microsensors Instruction Manual

	ISO-NOP007	ISO-NOP3005	ISO-NOP3020
Outside Diameter	7 µm	30 μm	30 µm
Available Length	2 mm	0.5 mm	2 mm
Response Time	< 3 seconds	< 3 seconds	< 3 seconds
Lowest Detection Limit/Range	0.5 nM	1 nM	1 nM
Nominal Sensitivity-New sensor	≥1 pA/nM	≥1.4 pA/nM	≥1.5 pA/nM
Baseline Drift	none	none	none
Poise Voltage	865 mV	865 mV	865 mV
Typical Quiescent Baseline Current, 25°C	300 pA	1000 pA	500 pA
Acceptable Baseline Range	200-1500 pA	150-3500 pA	500-5000 pA
Polarization Time	1+ hours	1+ hours	1+ hours