Analog Microforge

MF200-2

Options

Prices valid in USA, Canada, and PR only.

The MF200 Microforge is a versatile instrument designed specifically for the fabrication of glass micropipettes and other related tools. The system was developed in collaboration with Dr. Ming Li of the Department of Pharmacology, University of South Alabama. The MF200 is simple, reliable and economically priced.

Details

Sometimes the simplest designs work best

Features

  • Simple, reliable and economically priced
  • Analog temperature controller
  • W30S-LED microscope (optional)
  • Shown with MF200-FS foot switch (optional)

Options

Order code Power Microscope
MF200-1 110V Yes
MF200-2 220V Yes
MF200-M1 110V No
MF200-M2 220V No

Click here to view the current Data Sheet.     

Click to view the pullers, bevelers, microforge application guide to compare all the units.

Benefits

  • Includes 40x long-working distance objective and 10x eyepiece
  • Kohler illuminator and Abbe condenser for less glare and sharper images

Applications

  • Patch pipette tip polishing
  • Micropipette tip size reduction
  • Contact stretching in in vitro fertilization pipette production

40X LWD objective included

The MF200 system includes:

  • An easy to use analog temperature controller
  • A specially configured WPI model W30S-LED research grade compound microscope (optional)
  • 40x long-working distance objective
  • 10x eyepiece

40x magnification is essential when polishing pipettes as small as half a micron (0.5 µm) in diameter. Compared to a conventional 40x objective, the long working distance objective reduces the danger of damage to the pipette and/or objective lens during the polishing process.

Kohler illuminator

It is also the only commercial microforge using the Kohler illuminator and Abbe condenser for illumination. This provides less glare and sharper image of the pipette than frosted glass illuminator, which was used on all of the other commercial microforges.

Resources

MF200 Manual

The image below shows the Microforge kit.

The Microforge MF200 Startup Kit includes these elements

Video

DMF 1000: Setting up the heating filament on the microscope

 

Specifications

AC POWER MODULE 100-240 VAC 50/60 Hz
FILAMENTS (3) H2, H3, H4
FILAMENT ON Pushbutton Controlled or Optional Foot Switch Controlled
FILAMENT ADJUSTMENT ASSEMBLY For 40x and 25x Long-Working, Distance Objectives: mounts on objective
FILAMENT ADJUSTMENT ASSEMBLY: OBJECTIVE 40x Long-Working Distance (3 mm)
FILAMENT ADJUSTMENT ASSEMBLY: OPTIONAL 25x Long-Working Distance (5 mm)
EYEPIECE 10x (pair)
EYEPIECE: RETICLE (10x eyepiece only) (OPTIONAL) 1.25 µm/division (at 40x), 0-90º Angle at 5º/division
EYEPIECE: OPTIONAL EYEPIECE 15x (pair)
GLASS HOLDER Mounts on Microscope Stage
DIMENSIONS: Control Unit 4 x 7 x 1.875in. (10.2 x 17.8 x 4.8 cm)
SHIPPING WEIGHT 3 lb. (1.4 kg)
MICROSCOPE: Note See W30S-LED
MICROSCOPE: SHIPPING WEIGHT 16 lb. (7.3 kg)

Objectives Included

W30S-LED Microscope Objectives

  • DIN Plan with 30-year anti-fungal coating
  • 4X, 10X, 40X, 100XR (oil immersion)
  • Parfocal, parcentric, color-coded
  Numerical
Aperature		 Magnification Field of View Working
Distance
4X 0.1 40X 4.5mm 17.5mm
10X 0.25 100X 1.8mm 6.7mm
40X 0.65 400X 0.45mm ~2.9mm
100XR 1.25 1000X 0.18mm 0.18mm

Microforge LWD Objectives

    Numerical
Aperature		 Magnification Field of View Working
Distance
Included 40X 0.65 400X 0.45mm 3mm
Optional 25X 0.25 250X 0.72mm 5mm

References

Guillou, L., Babataheri, A., Puech, P.-H., Barakat, A. I., & Husson, J. (2016). Dynamic monitoring of cell mechanical properties using profile microindentation. Scientific Reports, 6, 21529. http://doi.org/10.1038/srep21529

Vasauskas, A. A., Chen, H., Wu, S., & Cioffi, D. L. (2014). The serine-threonine phosphatase calcineurin is a regulator of endothelial store-operated calcium entry. Pulmonary Circulation, 4(1), 116–27. http://doi.org/10.1086/675641

Pawlikowska-Pawlęga, B., Dziubińska, H., Król, E., Trębacz, K., Jarosz-Wilkołazka, A., Paduch, R., … Gruszecki, W. I. (2014). Characteristics of quercetin interactions with liposomal and vacuolar membranes. Biochimica et Biophysica Acta (BBA) - Biomembranes, 1838(1), 254–265. http://doi.org/10.1016/j.bbamem.2013.08.014

Koselski, M., Trebacz, K., & Dziubinska, H. (2013). Cation-permeable vacuolar ion channels in the moss Physcomitrella patens: a patch-clamp study. Planta, 238(2), 357–367. http://doi.org/10.1007/s00425-013-1902-4

Huang, C.-X. (2012). Amino acid substitutions in the pore affect the anomalous mole fraction effect of CaV1.2 channels. Molecular Medicine Reports. http://doi.org/10.3892/mmr.2012.1210

Hillsley, K., Lin, J.-H., Stanisz, A., Grundy, D., Aerssens, J., Peeters, P. J., … Stead, R. H. (2006). Dissecting the role of sodium currents in visceral sensory neurons in a model of chronic hyperexcitability using Na v 1.8 and Na v 1.9 null mice. The Journal of Physiology, 576(1), 257–267. http://doi.org/10.1113/jphysiol.2006.113597

Ouyang, W., & Hemmings, H. C. (2005). Depression by Isoflurane of the Action Potential and Underlying Voltage-Gated Ion Currents in Isolated Rat Neurohypophysial Nerve Terminals. Journal of Pharmacology and Experimental Therapeutics, 312(2).

Wang, X., Ponoran, T. A., Rasmusson, R. L., Ragsdale, D. S., & Peterson, B. Z. (2005). Amino Acid Substitutions in the Pore of the CaV1.2 Calcium Channel Reduce Barium Currents without Affecting Calcium Currents. Biophysical Journal, 89(3), 1731–1743. http://doi.org/10.1529/biophysj.104.058875

Zhuang, H., Bhattacharjee, A., Hu, F., Zhang, M., Goswami, T., Wang, L., … Li, M. (2000). Cloning of a T-Type Ca Channel Isoform in I n s u l i n-Secreting Cells. DIABETES, 49.