EVOM2 Cell Culture Cup Chambers for TEER Measurement

$2,250
$2,250

ENDOHM-12G

$2,250
$2,250
Size (mm)

Prices valid in USA, Canada, and PR only.

These Cell Culture Cup Chambers used in TEER measurement for removable culture cup systems using EVOM2 meters for endothelial and epithelial cell cultures.

NOTE: These EndOhms are for use with older EVOM™ meters. For EndOhms used with the new EVOM™ Manual (or EVOM3), see EVOM™ Electrodes for TEER.

EndOhm chambers provide reproducible resistance measurements of endothelial and epithelial monolayers in culture cups. Transfer cups from their culture wells to the EndOhm chamber for measurement rather than using hand-held electrodes. The chamber and the cap each contain a pair of concentric electrodes: a voltage-sensing silver/silver chloride pellet in the center plus an annular current electrode. The height of the top electrode can be adjusted to fit cell culture cups of different manufacture.

To learn more about our warranty options, click here.

Curious how EndOhm chamber improves reproducibility in TEER experiments?

Discover the benefits here!

Details

For TEER measurement of epithelial and endothelial cell cultures

Features

  • The new EndOhm chamber upper mount is made of polycarbonate and unaffected by alcohol
  • The glass chamber is easier to clean and more scratch resistant than the prior versions. The EndOhm is not recommended for incubator use due to risk of the glass cracking.
  • Adjustable apical electrode height
  • Crystal clear glass chamber allows visualization of apical electrode positioning
  • New insert holder with 120º tri-supports for three leg inserts
  • Three sizes cover a range of well cup sizes from a variety of manufacturers
  • Compatible with EVOM, EVOM2™ and Millicell® ERS and ERS2.  For EndOhms used with the new EVOM™ Manual (or EVOM3), see EVOM™ Electrodes for TEER.

To find the equivalent EndOhm chambers compatible with EVOM3 or EVOM™ Manual, visit the product page here.

 

Options

Order Code Description
ENDOHM-24G-SNAP EndOhm for 24mm and Costar Snapwell Culture Cups (6 wells per plate)
ENDOHM-12G EndOhm for 12mm Culture Cups (12 wells per plate)
ENDOHM-6G EndOhm for 6mm Culture Cups (24 wells per plate)


Benefits

  • Stability and reproducibility superior to the STX2 electrodes to 1% tolerance
  • Can be used with 6, 12 or 24 well plates with removable inserts
  • Symmetrical electrode pattern disperses test current uniformly
  • Tri-leg supports offer mechanical stability and the membrane is held parallel to the electrodes (G version)
  • Simple test procedure to verify electrode performance

Applications

  • TEER measurement for removable culture cup systems using EVOM2 meters for endothelial and epithelial cell cultures

EVOM-Manual-EndOhm

ENDOHMapplication

TEER measurement in individual cups

Using WPI’s EVOM2 resistance meter, Endohm chambers provide reproducible resistance measurements of endothelial and epithelial monolayers in culture cups. Transfer cups from their culture wells to the Endohm chamber for measurement rather than using hand-held electrodes. The chamber and the cap each contain a pair of concentric electrodes: a voltage-sensing silver/silver chloride pellet in the center plus an annular current electrode. The height of the top electrode can be adjusted to fit cell culture cups of different manufacture.

Make more precise measurements with Endohms

Endohm’s symmetrically opposing circular disc electrodes, situated above and beneath the membrane, allow a more uniform current density to flow across the membrane than with STX2 electrodes. The background resistance of a blank insert is reduced from 150 Ω (when using WPI’s hand-held STX2 electrodes) to less than 5 Ω. With Endohm’s fixed electrode geometry, variation of readings on a given sample is reduced from 10-30 Ω with STX2 electrodes (depending on the experience of the user) to 1-2 Ω. Compared with other resistance measurement methods, Endohm with EVOM2™ offers a much more convenient and economic solution to “leaky tissue” measurement. Because of the uniform density of the AC square wave current from EVOM2, errors owing to electrode polarization or membrane capacitance are largely eliminated. Endohm together with EVOM2 offers the most accurate and economical endothelial ohmmeter now available. To date, cups from Corning, Millipore, Nunc, Greiner and BD Falcon have been tested. Endohm chambers may be sterilized with EtO, alcohol or a bactericide; not autoclavable.

NOTE: EndOhm chambers have Ag/AgCl electrodes. If you are measuring for extended periods of time, you should consider any potential cytotoxic issue from long term exposure to silver to your cells.

Compatibility Charts

The ENDOHM-6G is compatible with the following chambers:

Corning  Millipore   Material Membrane Diameter (mm) Growth Surface Area (cm²) Membrane Pore Size (μm)
3470     6.5 0.33 0.4
3472 PITP01250   6.5 0.33 3.0
3413 PCF Insert   6.5 0.33 0.4
3415 PITP 01250
PCF Insert		   6.5 0.33 3.0
3421     6.5 0.33 5.0
3422 PIEP 01250
PCF Insert		   6.5 0.33 8.0
3495  PIHT12R48*
PET Insert		   6.5 0.33 0.4
  PIHA012 50 HA Insert 6.5 0.33 0.45
  PICM012 50 CM Insert 6.5 0.33 0.4
3496 PISP12R48*
PET Insert		   6.5 0.33 3.0
  PIRP12R48*
PET Insert		   6.5 0.33 1.0
  PIMP12R48*
PET Insert		   6.5 0.33 5.0
  PIEP12R48*
PET Insert		   6.5 0.33 8.0
  PIXP01250
PCF Insert		   6.5 0.33 12
  PIHP01250       1.0
  PITT01250       3.0

* The tri-supports overhang the chamber edge and the well cannot be held parallel to the electrodes.

Nunc Pore size (μm) Culture area (cm²)
140620 0.4 0.47
140627 3.0 0.47
140629 8.0 0.47

 

ThinCertTM Membrane material Pore size [µm] Pore density [cm-2] Optical membrane properties TC surface treatment/Sterile Multiwell plates/ThinCertTM per box
662640 PET 0.4 1 x 108 translucent +/+ 2/48
662641 PET 0.4  2 x 106 transparent +/+ 2/48
662610 PET 1.0  2 x 106 transparent +/+ 2/48
662630 PET 3.0  0.6 x 106 transparent +/+ 2/48
662631 PET 3.0  2 x 106 translucent +/+ 2/48
662638 PET 8.0  0.15 x 106 translucent +/+ 2/48

 

Millicell Pore size (μm) Qty/pk
MCHT24H48 0.4 48
MCRP24H48 1.0 48
MCSP24H48 3.0 48
MCMP24H48 5.0 48
MCEP24H48 8.0 48

 

BD Falcon Membrane material Pore size [µm] Pore density [cm-2] Optical membrane properties TC plate (#wells)
353095 PET 0.4 2.0 ± 0.2 x 106 transparent 24
353104 PET 1.0 1.6 ± 0.6 x 106 transparent 24
353096 PET 3.0 8 ± 2 x 105 transparent 24
353097 PET 8.0 6 ± 2 x 104 translucent 24
353495 PET 0.4HD 100 ± 10 x 106 translucent 24
353492 PET 3.0HD 2.0 ± 0.2 x 105 translucent 24

 

The ENDOHM-12G is compatible with the following chambers:

Corning Millipore Membrane Diameter (mm) Growth Surface Area (cm²) Membrane Pore Size (μm)
3401   12  1.12 0.4
3402 PITP01250 12   1.12 3.0
  PITT01250 12   1.12 3.0
3493   12 1.12 0.4
3494   12 1.12 3.0
3460 PIHT15R48*
PET Insert		 12 1.12 0.4
  PIRP15R48*
PET Insert		 12 1.12 1.0
3462 PISP15R48*
PET Insert		 12 1.12 3.0
  PIMP15R48*
PET Insert		 12 1.12 5.0
  PIEP30R48*
PIEP15R48*
PET Insert 		12 1.12 8.0

* The tri-supports legs must be balance correctly so that the filter is parallel to the electrodes.

Nunc Pore size (μm) Culture area (cm²)
140652 0.4 1.13
140654 3.0 1.13
140656 8.0 1.13

 

ThinCertTM Membrane material Pore size [µm] Pore density [cm-2] Optical membrane properties TC surface treatment/Sterile Multiwell plates/ThinCertTM per box
665640 PET 0.4 1 x 108 translucent +/+ 4/48
665641 PET 0.4  2 x 106 transparent +/+ 4/48
665610 PET 1.0  2 x 106 transparent +/+ 4/48
665630 PET 3.0  0.6  x 106 transparent +/+ 4/48
665631 PET 3.0  2 x 106 translucent +/+ 4/48
665638 PET 8.0  0.15 x 106 translucent +/+ 4/48

 

Millicell Pore size (μm) Qty/pk
MCHT12H48 0.4 48
MCRP12H48 1.0 48
MCSP12H48 3.0 48
MCMP12H48 5.0 48
MCEP12H48 8.0 48

 

BD Falcon Membrane material Pore size [µm] Pore density [cm-2] Optical membrane properties TC plate (#wells)
353180 PET 0.4 2.0 ± 0.2 x 106 transparent 12
353103 PET 1.0 1.6 ± 0.6 x 106 transparent 12
353181 PET 3.0 8 ± 2 x 105 transparent 12
353182 PET 8.0 6 ± 2 x 104 translucent 12
353494 PET 0.4HD 100 ± 10 x 106 translucent 12
353292 PET 3.0HD 2.0 ± 0.2 x 105 translucent 12

 

The ENDOHM-24SNAP is compatible with the following chambers:

Corning Millipore  Membrane Material Pore Size (µm)
3407   Polycarbonate 0.4
3801   Polycarbonate 0.4
    Polycarbonate 3.0
3412 PIHT30R48* Polycarbonate 0.4
3414   Polycarbonate 3.0
  PITT03050 Polycarbonate 3.0
3428   Polycarbonate 8.0
3450   Polyester 0.4
3452   Polyester 3.0 
3491   Collagen 0.4
3492   Collagen 3.0
  PICMORG50 Organotypic Insert 0.4
  PIHA03050 HA Insert 0.45
  PIHP03050 PCF Insert 0.4
  PICM03050 HA mixed cellulose esters 0.4 
  PIHT30R48* PET Insert 0.4
  PIRP30R48* PET Insert 1.0
  PISP30R48* PET Insert 3.0
  PIMP30R48* PET Insert 5.0
  PIEP30R48* PET Insert 8.0

* The tri-support legs must be balanced correctly so that the filter is parallel to the electrodes.

Nunc Pore size (μm) Culture area (cm²)
140640 0.4 3.14
140642 3.0 3.14
140644 8.0 3.14
140660 0.4 4.1
140663 3 4.1
140668 8 4.1

 

ThinCertTM Membrane material Pore size [µm] Pore density [cm-2] Optical membrane properties TC surface treatment/Sterile Multiwell plates/ThinCertTM per box
657640 PET 0.4 1 x 108 translucent +/+ 4/24
657641 PET 0.4  2 x 106 transparent +/+ 4/24
657610 PET 1.0  2 x 106 transparent +/+ 4/24
657630 PET 3.0  0.6 x 106 transparent +/+ 4/24
657631 PET 3.0  2 x 106 translucent +/+ 4/24
657638 PET 8.0  0.15 x 106 translucent +/+ 4/24

 

Millicell Pore size (μm) Qty/pk
MCHT06H48 0.4 48
MCRP06H48 1.0 48
MCSP06H48 3.0 48
MCMP06H48 5.0 48
MCEP06H48 8.0 48

 

BD Falcon Membrane material Pore size [µm] Pore density [cm-2] Optical membrane properties TC plate (#wells)
353090 PET 0.4 2.0 ± 0.2 x 106 transparent 6
353102 PET 1.0 1.6 ± 0.6 x 106 transparent 6
353091 PET 3.0 8 ± 2 x 105 transparent 6
353093 PET 8.0 6 ± 2 x 104 translucent 6
353493 PET 0.4HD 100 ± 10 x 106 translucent 6
353092 PET 3.0HD 2.0 ± 0.2 x 105 translucent 6

 

Frequently Asked Questions

Resources

Endohm Instruction Manual

Endohm Data Sheet

Endohm Sell Sheet

EndOhm-6G (EVM-EL-03-01-01) Compatibility Charts

EndOhm-12G (EVM-EL-03-01-02) Compatibility Charts

EndOhm-24G-SNAP (EVM-EL-03-01-03) Compatibility Charts

Video

 

FAQ

Troubleshooting Unstable Resistance Readings from an ENDOHM

One of our frequently asked questions (FAQs) concerns TEER measurements with an EndOhm. If the resistance readings from your EndOhm don't stabilize, you may need to do some troubleshooting.

Test the manual EVOM™ meter: First, test your EVOM™ meter. The 1000Ω test resistor (WPI # 91750) can be used for this purpose. Insert the RJ-11 plug at the end of the test resistor into the Input port on the meter. Set the Function Switch to Ohms. Disconnect the EVOM™ meter from the charger and turn the Power on (I). The meter should display 1000Ω. If not, adjust the R ADJ screw with a small slot-head screwdriver until the meter shows a reading of 1000Ω. If the EVOM™ meter reads 1000 ± 2-3 ohms, and the reading remains stable, then the EVOM™ meter is working correctly.

Test the EndOhm: Next, test the EndOhm. You can still test the EndOhm qualitatively by exposing it to different KCl concentrations. The readings should always be a stable, lower TEER value at higher concentrations, and a higher but potentially less stable value at lower concentrations. In general, if the TEER reading is falling, it means that the current is finding an alternative path of lower resistance than through the media alone, or the preparation is somehow adopting a charge. If the problem is truly in the EndOhm, it will typically be caused by a leak of culture media beneath the electrode surfaces, where it can attack wire bonds to the Ag/AgCl disks. A delayed reaction may take time for the media to creep into very fine fissures where the glue bond has lost seal integrity. If the TEER reading continually drifts downward well below the expected value, then the EndOhm most likely has a leak at the electrode bond or corrosion somewhere in the current or voltage pathways. If the EndOhm has developed fine fissures, it needs to be replaced.

References

Sheller, R. A., Cuevas, M. E., & Todd, M. C. (2017). Comparison of transepithelial resistance measurement techniques: Chopsticks vs. Endohm. Biological Procedures Online, 19, 4. http://doi.org/10.1186/s12575-017-0053-6 

Srinivasan, B., Kolli, A. R., Esch, M. B., Abaci, H. E., Shuler, M. L., & Hickman, J. J. (2015). TEER measurement techniques for in vitro barrier model systems. Journal of Laboratory Automation, 20(2), 107–26. http://doi.org/10.1177/2211068214561025

TORRES, R., PIZARRO, L., CSENDES, A., GARCÍA, C., LAGOS, N., Pasdar, M., … Roskelley, C. (2007). GTX 2/3 EPIMERS PERMEATE THE INTESTINE THROUGH A PARACELLULAR PATHWAY. The Journal of Toxicological Sciences, 32(3), 241–248. http://doi.org/10.2131/jts.32.241 

Patil, R. V., Han, Z., Yiming, M., Yang, J., Iserovich, P., Wax, M. B., & Fischbarg, J. (2001). Fluid transport by human nonpigmented ciliary epithelial layers in culture: a homeostatic role for aquaporin-1. American Journal of Physiology - Cell Physiology, 281(4).
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