Fiber Optic Oxygen Meter for Minisensors

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Features

• Oxygen is not consumed during the experiment
• Immune to electrical and magnetic interference
• Excellent long-term stability
• No lengthy polarization necessary (like Clark-type O2 electrodes)
• Fast response time < 0.5 s for MicroTip sensors
• Probe size of MicroTip sensors as small as 50 µm
• Measurement is feasible in dry gas
• Optical isolation of sensor tip available for fluorescent or photosynthetically active samples
• The sensors are not included within the system

Benefits

• Unaffected by light source stability and intensity fluctuations, because it is based on luminescence lifetime detections.
• Compact, portable meters may be used inside or outside

Applications

• Process control like bottling plant in breweries and quality control of packages (OxyMini)
• Biotechnology like control of cell culture media and non-invasive control of bioreactors (OxyMini)
• Implantation of oxygen sensors into soil and trees (OxyMini)
• Oxygen profiles of marine sediment, soils, or tissue (OxyMicro)
• Implantation in living tissue like heart or muscle tissue (OxyMicro)
• Control of cell culture media in Biotechnology (OxyMicro)

Unaffected by light source stability/intensity

Conventional fiberoptic oxygen sensor systems based on intensity measurements are limited in their accuracy by light source stability and ambient light fluctuations. Using a luminescence lifetime detection, measurements are not affected by light source stability, intensity fluctuations caused by fiber bending or changes of the optical properties of the sample (turbidity, refractive index, coloration, etc.).

Calibration: The sensors can be calibrated by a simple two point calibration, 100% air-saturation and 0% air saturation.

Compact, portable meters, inside and outside

The OxiMini and OxyMicro fiber optic oxygen meters are compact, easy to transport. Designed for in/outdoor use, they can be connected to a PC via a RS232 interface. Data can be visualized, analyzed and stored with supplied software. A full range of sensors are available.

OxyMini systems

The OxyMini is a one channel fiber optic O₂ meter for fiber optic O₂ minisensors. These sensors are based on 2 mm polymer optical fibers and have a length of 2.5 m.

MiniTip – This dipping probe (501641) has a tip diameter of 4mm and consists of a polymer optical fiber with an O2 sensitive coating. Its range is 0–100%. It has a response time (t90) of ~ 40 s.

MiniFlow – The MiniFlow probe (501642) is a miniaturized fiber optic chemical sensor integrated in a standard T-shape flow through cell which can be easily connected via Luer-Lock adapters to external tubings. Liquids can be pumped through the cell. It has a response time (t90) of approximately 40 s and an excellent long-term stability.

MiniFoil – Sensor material on a 1cm2 support disk made of polyester can be glued inside glass vials. Measure oxygen concentration non-invasively, non-destructively from outside through the wall of the vial. Illuminate the sensor foil with a plastic fiber optic cable (501644, 01645). The wall of the flask must be transparent/non-fluorescent. (Response time (t90) of ~50s.) The material can be implanted into animal tissues or custom-made housings. 

The OxyMini system is optimized for process control and biotechnology applications. The measurement principle of the sensor system is based on the detection of oxygen concentration as a function of luminescence lifetime either in dissolved or gaseous phase environments. The fiber optic oxygen meter is compact and easy to transport. It was designed for in/outdoor use and can be connected to a PC via a RS232 interface. Data can be visualized, analyzed and stored with the supplied software. A full range of sensors covering most biomedical applications are available.

Measurement Principle

Conventional fiber-optic oxygen sensor systems based on intensity measurements are limited in their accuracy by light source stability and ambient light fluctuations. Using a luminescence lifetime detection, measurements are not affected by light source stability, intensity fluctuations caused by fiber bending or changes of the optical properties of the sample (turbidity, refractive index, coloration, etc.). These advantages make WPI's OxyMini and OxyMicro the most advanced and reliable fiber-optic oxygen system available.

Calibration

The sensors can be calibrated by a simple two point calibration, 100% air-saturation and 0% air saturation.

OxyMini Instruction Manual

	MiniTip	MiniFlow	MiniSpot	MicroTip	MicroFlow	MicroImplant
MEASURE RANGE DISSOLVED/GASEOUS	0-45 ppm, 0-100% 0-760 mmHg	0-45 ppm, 0-100% 0-760 mmHg	0-45 ppm, 0-100% 0-760 mmHg	0-45 ppm, 0-100% 0-760 mmHg	0-45 ppm, 0-100% 0-760 mmHg	0-45 ppm, 0-100% 0-760 mmHg
RESPONSE TIME [T₉₀] DISSOLVED/GASEOUS	40 s 10 s	40 s 10 s	40 s 10 s	< 2 s < 0.5 s	< 2 s < 0.5 s	< 2 s < 0.5 s
STERILIZATION ETOH, H₂O₂ AUTOCLAVABLE*	Y N	Y Y	Y Y	Y N	Y Y	Y Y
DRIFT**	< 0.1%	< 0.1%	< 0.1%	< 0.3%	< 0.3%	< 0.3%
ACCURACY***	0.2%
RESOLUTION***	2.75 ±0.01 ppm, 9.00 ±0.05 ppm, 220 ±0.15 ppm, 45.0 ±0.25 mmHg, 150 ±0.75 mmHg, 375 ±2.6 mmHg
TEMP RANGE	-10°C to 50°C
PROBE ASSY LENGTH	2.5 m
*130°C, 1.5 atm  **100,000 data points, 20°C  ***20°C