Fiber Optic Oxygen Meter for Microsensors

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Features

• Oxygen is not consumed during the experiment
• Immune to electrical and magnetic interference
• Excellent long-term stability
• No lengthy polarization necessary (like Clark-type O2 electrodes)
• Fast response time < 0.5 s for MicroTip sensors
• Probe size of MicroTip sensors as small as 50 µm
• Measurement is feasible in dry gas
• Optical isolation of sensor tip available for fluorescent or photosynthetically active samples
• The sensors are not included within the system

Benefits

• Unaffected by light source stability and intensity fluctuations, because it is based on luminescence lifetime detections.
• Compact, portable meters may be used inside or outside

Applications

• Process control like bottling plant in breweries and quality control of packages (OxyMini)
• Biotechnology like control of cell culture media and non-invasive control of bioreactors (OxyMini)
• Implantation of oxygen sensors into soil and trees (OxyMini)
• Oxygen profiles of marine sediment, soils, or tissue (OxyMicro)
• Implantation in living tissue like heart or muscle tissue (OxyMicro)
• Control of cell culture media in Biotechnology (OxyMicro)

Unaffected by light source stability/intensity

Conventional fiberoptic oxygen sensor systems based on intensity measurements are limited in their accuracy by light source stability and ambient light fluctuations. Using a luminescence lifetime detection, measurements are not affected by light source stability, intensity fluctuations caused by fiber bending or changes of the optical properties of the sample (turbidity, refractive index, coloration, etc.).

Calibration: The sensors can be calibrated by a simple two point calibration, 100% air-saturation and 0% air saturation.

Compact, portable meters, inside and outside

The OxiMini and OxyMicro fiber optic oxygen meters are compact, easy to transport. Designed for in/outdoor use, they can be connected to a PC via a RS232 interface. Data can be visualized, analyzed and stored with supplied software. A full range of sensors are available.

OxyMicro systems

OxyMicro is a one channel O2 meter for fiber optic O2 microsensors.

MicroTip – The MicroTip (501656) is a needle-type (27 ga.) oxygen micro sensor designed for applications where a small tip size (50 µm) and fast response time (t90) of 1 s are required. The oxygen sensitive sensor tip consists of 140 µm fiber tapered to a 50 µm tip. Housed inside a stainless steel needle 22 mm long and 0.4 mm diameter, it can penetrate a septum rubber or similar material. They are ideal for oxygen profiling in sediments and biofilms.

MicroFlow – The MicroFlow oxygen sensor (501657) is a miniaturized fiber optic chemical sensor optimized for fast response time (t90 < 1 sec in gases, < 5 sec in liquids). The tiny probe has a tip size of 50 µm and is integrated in a T-shape flow cell for easy connection via Luer-Lock adapters to external tubings. Liquids (like water, blood, etc.) can be pumped through the cell.

MicroImplant – The MicroImplant oxygen sensor (501658) is an implantable probe (IMP) with a probe tip size 50 µm, an exposed fiber length of 5 mm and a jacket diameter of 900 µm. It was successfully implanted in crabs, fishes and soil.

OxyMicro Instruction Manual

	MiniTip	MiniFlow	MiniSpot	MicroTip	MicroFlow	MicroImplant
MEASURE RANGE DISSOLVED/GASEOUS	0-45 ppm, 0-100% 0-760 mmHg	0-45 ppm, 0-100% 0-760 mmHg	0-45 ppm, 0-100% 0-760 mmHg	0-45 ppm, 0-100% 0-760 mmHg	0-45 ppm, 0-100% 0-760 mmHg	0-45 ppm, 0-100% 0-760 mmHg
RESPONSE TIME [T₉₀] DISSOLVED/GASEOUS	40 s 10 s	40 s 10 s	40 s 10 s	< 2 s < 0.5 s	< 2 s < 0.5 s	< 2 s < 0.5 s
STERILIZATION ETOH, H₂O₂ AUTOCLAVABLE*	Y N	Y Y	Y Y	Y N	Y Y	Y Y
DRIFT**	< 0.1%	< 0.1%	< 0.1%	< 0.3%	< 0.3%	< 0.3%
ACCURACY***	0.2%
RESOLUTION***	2.75 ±0.01 ppm, 9.00 ±0.05 ppm, 220 ±0.15 ppm, 45.0 ±0.25 mmHg, 150 ±0.75 mmHg, 375 ±2.6 mmHg
TEMP RANGE	-10°C to 50°C
PROBE ASSY LENGTH	2.5 m
*130°C, 1.5 atm  **100,000 data points, 20°C  ***20°C