The Role of Aladdin Syringe Pumpsin Organ-on-Chip Applications

Among the vast landscape of cell lines explored in pre-clinical organ-on-a-chip (OOC) spaces, there are many high, medium, and entry-level pumping solutions available. While various high-end solutions offering the utmost flow accuracy (within 1%) over an extended observation period may be attractive, this level of precision may not be critical to achieve confluency across all cell lines, opening doors for promising results to many end-users seeking simplicity in their workflows.

Explore the many ways WPI’s Aladdin Syringe Pump line has served both the Lab- and Organ-on-Chip spaces:

  1. Abbasi, R., LeFevre, T. B., Benjamin, A. D., Thornton, I. J., & Wilking, J. N. (2021). Coupling fluid flow to hydrogel fluidic devices with reversible "pop-it" connections. Lab on a Chip, 21(10), 2050–2058. https://doi.org/10.1039/d1lc00135c
  2. Busek, M., Nøvik, S., Aizenshtadt, A., Amirola-Martinez, M., Combriat, T., Grünzner, S., & Krauss, S. (2021). Thermoplastic Elastomer (TPE)-Poly(Methyl Methacrylate) (PMMA) Hybrid Devices for Active Pumping PDMS-Free Organ-on-a-Chip Systems. Biosensors, 11(5), 162. https://doi.org/10.3390/bios11050162
  3. Carvalho, D. J., Kip, A. M., Romitti, M., Nazzari, M., Tegel, A., Stich, M., Krause, C., Caiment, F., Costagliola, S., Moroni, L., & Giselbrecht, S. (2023). Thyroid-on-a-Chip: An Organoid Platform for In Vitro Assessment of Endocrine Disruption. Advanced healthcare materials, 12(8), e2201555. https://doi.org/10.1002/adhm.202201555
  4. Chen, Z., Huang, J., Zhang, J., Xu, Z., Li, Q., Ouyang, J., Yan, Y., Sun, S., Ye, H., Wang, F., Zhu, J., Wang, Z., Chao, J., Pu, Y., & Gu, Z. (2023). A storm in a teacup -- A biomimetic lung microphysiological system in conjunction with a deep-learning algorithm to monitor lung pathological and inflammatory reactions. Biosensors & Bioelectronics, 219, 114772. https://doi.org/10.1016/j.bios.2022.114772
  5. Conde, A. J., Keraite, I., Ongaro, A. E., & Kersaudy-Kerhoas, M. (2020). Versatile hybrid acoustic micromixer with demonstration of circulating cell-free DNA extraction from sub-ml plasma samples. Lab on a Chip, 20(4), 741–748. https://doi.org/10.1039/c9lc01130g
  6. Deli, M.A., Porkoláb, G., Kincses, A., Mészáros, M., Szecskó, A., Kocsis, A.E., Vigh, J.P., Valkai, S., Veszelka, S., Walter, F.R., & Dér, A. Lab-on-a-chip models of the blood–brain barrier: evolution, problems, perspectives. Lab on a Chip, 24(5), 1030-1063. https://doi.org/10.1039/D3LC00996C
  7. Elitas, M., Dhar, N., & McKinney, J. D. (2021). Revealing Antibiotic Tolerance of the Mycobacterium smegmatis xanthine/uracil permease mutant using microfluidics and single-cell analysis. Antibiotics (Basel), 10(7), 794. https://doi.org/10.3390/antibiotics10070794
  8. Liu, E.Y., Jung, S., Weitz, D.A., Yi, H., & Choi, C.H. (2018). High-throughput double emulsion-based microfluidic production of hydrogel microspheres with tunable chemical functionalities toward biomolecular conjugation. Lab on a Chip, 18(2), 323-334. https://doi.org/10.1039/C7LC01088E
  9. Mazzarda, F., D'Elia, A., Massari, R., De Ninno, A., Bertani, F. R., Businaro, L., Ziraldo, G., Zorzi, V., Nardin, C., Peres, C., Chiani, F., Tettey-Matey, A., Raspa, M., Scavizzi, F., Soluri, A., Salvatore, A. M., Yang, J., & Mammano, F. (2020). Organ-on-chip model shows that ATP release through connexin hemichannels drives spontaneous Ca2+ signaling in non-sensory cells of the greater epithelial ridge in the developing cochlea. Lab on a Chip, 20(16), 3011–3023. https://doi.org/10.1039/d0lc00427h
  10. McMillan, K.S., Boyd, M., & Zagnoni, M. (2016). Transitioning from multi-phase to single-phase microfluidics for long-term culture and treatment of multicellular spheroids. Lab on a Chip, 16(18), 3548-3557. https://doi.org/10.1039/C6LC00884D
  11. Nguyen, A., Brandt, M., Muenker, T. M., & Betz, T. (2021). Multi-oscillation microrheology via acoustic force spectroscopy enables frequency-dependent measurements on endothelial cells at high-throughput. Lab on a Chip, 21(10), 1929–1947. https://doi.org/10.1039/d0lc01135e
  12. Protopapa, G., Bono, N., Visone, R., D'Alessandro, F., Rasponi, M., & Candiani, G. (2023). A new microfluidic platform for the highly reproducible preparation of non-viral gene delivery complexes. Lab on a Chip, 23(1), 136-145. https://doi.org/10.1039/D2LC00744D
  13. Stanley, C.E., Shrivastava, J., Brugman, R., Heinzelmann, E., van Swaay, D., & Grossmann, G. (2018). Dual-flow-RootChip reveals local adaptations of roots towards environmental asymmetry at the physiological and genetic levels. New Phytol, 217(3): 1357-1369. https://doi.org/10.1111/nph.14887
  14. Wang, Y., Deng, R., Yanga, L., & Bain, C.D. (2019). Fabrication of monolayers of uniform polymeric particles by inkjet printing of monodisperse emulsions produced by microfluidics. Lab on a Chip, 19(18), 3077-3085. https://doi.org/10.1039/C9LC00588A
  15. Wang, H., Enders, A., Preuss, J. A., Bahnemann, J., Heisterkamp, A., & Torres-Mapa, M. L. (2021). 3D printed microfluidic lab-on-a-chip device for fiber-based dual beam optical manipulation. Scientific Reports, 11(1), 14584. https://doi.org/10.1038/s41598-021-93205-9
  16. Zecong, F., Ding, Y., Zhang, Z., Wang, F., Wang, Z., Wange, H., & Pan, T. (2020). Digital microfluidic meter-on-chip. Lab on a Chip, 20(4), 722-733. https://doi.org/10.1039/C9LC00989B
  17. Zhuang, Y., Cheng, S., Kovalchuk, N.M., Simmons, M.J., Matar, O.K., Guo, Y., & Arcucci, R. (2022). Ensemble latent assimilation with deep learning surrogate model: application to drop interaction in a microfluidics device. Lab on a Chip, 22(17), 3187-3202. https://doi.org/10.1039/D2LC00303A