Microscope Kit Includes:
- Simple add-on polarizer
- Analyzer slider with rotating mount (360°rotatable)
- Compensator, λ, first order red
Background Information
What Does a Polarizer Do?
Polarizers, also known as polarizing filters, are used in polarized light microscopes to select plane-polarized light from natural or unpolarized light. They are placed between the light source and the sample to convert the light into a single direction of vibration. When the polarized light hits a doubly refracting specimen, it generates two wave components, called ordinary and extraordinary light rays, that are at right angles to each other. These waves pass through the specimen in different phases, which can improve imaging of certain materials, such as collagen.
What Does an Analyzer Slider Do?
An analyzer slider rotates to adjust the polarization of light that passes through an object, and reaches the eyepiece, resulting in maximum image contrast. The analyzer is a second filter that blocks out all but one polarization of light.
What Does a Compensator Do?
A first order red (incident wave plate) compensator, also known as a first order (full wave) retardation plate, is a versatile tool used in polarized light microscopy:
- Estimate optical path differences: Can estimate optical path differences ranging from a fraction of a wavelength to several wavelengths
- Determine optical sign: Can determine the optical sign (positive or negative) with birefringence (double refraction)
- Enhance contrast: Can enhance contrast in weakly birefringent specimens
- Locate interference colors: Can be used to locate interference colors and optic figures in birefringent materials
