SUMILON Companion Plate for 24 Well Cell Culture Inserts, Pkg of 12

Background & Plate Description

Permeable membrane cell culture inserts are normally used to grow cells to evaluate cellular functions, such as barrier formation, permeability, cell migration, and angiogenesis. During the maintenance of these cultures on cell culture insert plates, the media needs to be changed with fresh media and samples may need collected from apical (top of the insert) and basolateral (bottom of the insert) sides of the cell cultures.

The access to the basolateral compartment to replace media or collect samples could be challenging in standard insert plates, since the gap between the well of most standard 24-well plate and the insert is insufficient to use a pipette or aspirator without risking possible damage to the cell layer.

To conduct the media exchange or sample collection, inserts must be lifted one at a time, using tweezers or other tools, which is a labor-intensive process.  The 24-well SUMILON Companion Plate allows easy media exchange and sampling with cell culture inserts, overcoming tedious individual insert lifting and mitigating the risk of damaging samples and facilitating automation and high throughput analysis with cell culture inserts (e.g., automated TEER measurement).

 

Features

• These SUMILON Companion Plates are compatible with cell culture inserts from different manufacturers (e.g., Corning, Millipore, Grenier, Falcon, and others).
• The Companion Plates secure cell culture inserts in place with the designed notch at the well aperture, allowing you to tilt the plates and the inserts for manual or automated handling.
• The wells of these plates are shaped to hold the cell culture inserts firmly, so their positions are not altered during media exchange. The micro-steps on the inner sides of the wells prevent the rise in liquid level that can occur due to capillary action caused by dimension difference in the gap between the inserts and wells. And, a groove on the well wall aids in draining the culture medium.
• Medium exchange and sampling can be performed from the plate through a port adjoining the main well (“the Port”), without lifting the inserts. The port also provides sufficient space for the electrode of a TEER measurement instrument.
• The well bottom, including the port area, is cell culture-treated and offers a culture area comparable to conventional 24-well plates (187.5 mm²), enabling co-culture in both the insert and the well bottom of the plate.
• The space surrounding each well is prepared to accommodate sterilized water, which can reduce the drying out of the culture medium.
• Package of 12 plates, individually wrapped and sterile.

 

Benefits

• SUMILON Companion Plates minimize or eliminate chances of damaging the cellular layer by keeping cell culture inserts secured in place even during media change or sample collection or plate tilting.
• These plates minimize the effect of capillary action.
• Companion Plates make sample collection easy and convenient.
• Use of these plates saves experimentation time by eliminating the requirement of lifting individual inserts to change media or for basolateral sample collection.
• The Companion Plates allow cell culture inserts to be used with automation and enable high throughput analysis. Automated TEER measurement can be performed using an EVOM™ Auto, 24-well cell culture inserts and a 24-well SUMILON Companion Plate.
• The Companion Plates for 24-well inserts offer robustness to drug permeability assays by simplifying media exchange and test compound recovery, both manually and in laboratory automation for pharmaceutical drug discovery and development.
  
  

Applications

SUMILON Companion Plates are suitable for various cell culture insert experiments, including:

• Automated TEER Measurement
• Permeability Assay
• Toxicity Testing
• Cell Migration and Invasion Assays
• Co-Culture Studies

SUMILON Companion Plates are ideal for automated TEER measurement of commonly used 24-well hanging cell culture insert samples using WPI's EVOM™ Auto.

 

SUMILON Companion Plate Instruction Manual

SUMILON Companion Plate Sell Sheet

SUMILON Companion Plate Infographic

Specifications

Type	Descriptions
Packaging	Case of 12 Plates
Storage	Room Temperature
Individual Plates Sterile Packaging	Yes
Sterilization	Radiation
Expiration	3 Years from Date of Manufacture

Compatible Inserts

The 24-well hanging cell culture inserts from following manufacturers are compatible with SUMILON Companion Plates and allows automated TEER Measurement with WPI’s EVOM™ Auto.

• Corning Transwell^®
• FALCON^® CelCultureInserts 
• SABEU celQART^®
• KANTO CHEMICAL Vitrigel^®
• Greiner Bio-one ThinCert^® 
• MERCK MILLIPORE Millicell^®
• MatTek PermaCell