Set of 11 Calcium Buffers

$275
$275

CALBUF-2

Prices valid in USA, Canada, and PR only.

Use with calcium fluorescent indicators

CALBUF-2 is especially suitable for calibrating fluorescent Ca++ indicators. It provides eleven buffer standards in the 10-4 to 10-8 M Ca++ range, whereas other commonly used fluorescent Ca++ indicators have the apparent Kd in the range of 100 to 300 nM. As with any ionic sensitive indicator, the sensitivity range of these indicators is about 1.0 log unit above and below the Kd. CALBUF-2 provides seven calibration points in this sensitivity range. It has an osmolarity of 0.305, which is isotonic with most mammalian cells.

Details

Features

  • Use with calcium florescent indicators (KWIKCAL-2)
  • 20 mL of each buffer

 

Shelf life

Limited shelf life; use within 30 days.

 

Concentrations

1x10-8, 4x10-8, 1x10-7, 2.5x10-7, 5x10-7, 7.5x10-7, 1x10-6, 4x10-6, 1x10-5, 4x10-5, and 1x10-4 M at 20°C. Ionic strength: 0.150 M. 11 bottles, 20 mL each.

Resources

CALBUF2 Instruction Manual

References

Milán, A. F., Rincón, O. A., Arango, L. B., Reutovich, A. A., Smith, G. L., Giraldo, M. A., … Calderón, J. C. (2021). Calibration of mammalian skeletal muscle Ca2+ transients recorded with the fast Ca2+ dye Mag-Fluo-4. Biochimica et Biophysica Acta - General Subjects, 1865(9). https://doi.org/10.1016/j.bbagen.2021.129939

D.D. McKemy, W. Welch, J.A. Airey, J.L.Sutko "Concentrationsof caffeine greater than 20 mM increase the indo-1 fluorescence ratio in a Ca2+- independent manner" Annals of Botany 86. 2000: 687-703
Set of 11 Calcium Buffers

$275.00

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