Biochips For Cell Adhesion & Cell Culture Under Flow

Vena8 Fluoro+ Biochip

Cell-Ligand / Cell-Protein adhesion

Each biochip contains 8 capillaries in parallel which can be coated with different adhesion molecules for cell receptor-ligand (i.e. cell-matrix interactions) studies under shear flow.  

These biochips are compatible with confocal microscopy.

• Under shear flow
• Disposable biochip
• Cell adhesion, rolling assays on ligand/protein coated channels
• Ideal for platelet aggregation & thrombosis assays

Vena8 Fluoro+ Biochip Technical Notes

Vena8 Fluoro+ Biochip Protocol Notes

Vena8 Endothelial+ Biochip

Cell culture & cell-cell adhesion

Each biochip contains 8 capillaries in parallel which can be seeded with endothelial cells for culture of 8 monolayers in parallel and subsequent study of cell-cell interaction studies under shear flow.

• Under shear flow
• Disposable biochip
• Cell culture of endothelial cell monolayers in microchannel flow chambers
• Cell-cell adhesion shear flow assays

Vena8 Endothelial+ Biochip Technical Notes

Vena8 Endothelial+ Biochip Protocol Notes

Vena8 Biochip With Glass Coverslips

Cell-Ligand & cell-cell adhesion

• Under shear flow
• Disposable biochip
• Flexibility with glass coverslip supplied unattached.
• Glass coverslips may be treated with chelated Cu2+ ion tethered to the high density PEG-coating

Options:

• Coverslip: 

• Glass coverslip adhered

• Glass coverslip not adhered

• Adhesive layer adhered

• Surface treatment of chip:

  • Not treated

  • Treated with Cu2+ histidine tag

  • Treated with NHS leaving group on PEG brush

• Suitable for flow rates:

  • • Low Flow rates (1600 µm x 160 µm channel size)

    • High Flow rates (800 µm x 80 µm channel size)

Vena8 Biochip With Glass Coverslips Technical Notes

Vena8 Biochip With Glass Coverslips Low Shear Flow Rates

Vena8 Biochip With Glass Coverslips High Shear Flow Rates

Vena Delta Y1 Biochip

The VenaDeltaY1 biochips contain branching microchannels for dual flow / dual injection of samples.  Ideal for studying chemotactic gradients, dual flow, multilaminar flow & diffusion.

Each biochip contains 4 Y-shaped capillaries in parallel which can be coated with different adhesion molecules for cell receptor-ligand (i.e. cell-matrix interactions) studies under shear flow with dual-injection (chemotaxis experiments) or for investigation of thrombi formation at the Y-channel branch.

• Branching Y-junction at input port
• Mimic branching microvascular networks
• Chemotaxis assays, dual flow, multilaminar flow & diffusion
• Choice of channel size 400 µm (width) x 100 µm (depth) or 800 µm (width) x 120 µm (depth)

Vena8 Delta Y BiochipTechnical Notes

Vena Delta Y2 Biochip

The VenaDeltaY2 biochips contain branching microchannels for dual flow / dual injection of samples.  Ideal for studying chemotactic gradients, dual flow, multilaminar flow & diffusion.

Each biochip contains 4 Y-shaped capillaries at both ends in parallel which can be coated with different adhesion molecules for cell receptor-ligand (i.e. cell-matrix interactions) studies under shear flow with dual-injection (chemotaxis experiments) or for investigation of thrombi formation at the Y-channel branch.

• Branching Y-junction at input & exit ports
• Mimic branching microvascular networks
• Chemotaxis assays, dual flow, multilaminar flow & diffusion
• Channel sizes:  600 µm (width) x 100 µm (depth)

Vena8 Delta Y BiochipTechnical Notes

Vena Delta T Biochip

The VenaDeltaT biochip contains 4 T-junction shaped capillaries in parallel which can be coated with different adhesion molecules for cell receptor-ligand (i.e. cell-matrix interactions) studies under shear flow with dual-injection (chemotaxis experiments) or for investigation of thrombi formation at the T-channel branch.

• 4 T-junction shaped capillaries
• Channel sizes:  400 µm (width) x 100 µm (depth)

Vena Delta W Biochip

The VenDeltaW biochip contains 2 W-junction shaped capillaries and 1 Y-junction shaped capillary in the centre which can be coated with different adhesion molecules for cell receptor-ligand (i.e. cell-matrix interactions) studies under shear flow with dual-injection (chemotaxis experiments) or for investigation of thrombi formation at the W or Y-channel branch.

• 2 W-junction shaped capillaries
• 1 Y-junction shaped capillary
• Channel sizes:  400 µm (width) x 100 µm (depth)