{"title":"Pressure Upgrades","description":"\u003cp\u003e\u003cspan\u003ePressurized Superfusion, for faster switching and steady flow, and fine microliter perfusion and microinjecting\u003c\/span\u003e\u003c\/p\u003e\n\u003cdiv class=\"fusion-title title fusion-title-1 fusion-sep-none fusion-title-text fusion-title-size-two\"\u003e\n\u003ch2 class=\"title-heading-left\"\u003ePrecise control for whole-cell or single-cell superfusion.\u003c\/h2\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"fusion-text fusion-text-1\"\u003e\n\u003cp\u003e\u003cimg src=\"https:\/\/www.wpi-europe.com\/images\/content\/Pressure.png\" alt=\"\" width=\"650\" height=\"648\"\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"fusion-text fusion-text-2\"\u003e\n\u003ch3\u003ePerfusion Pressure Upgrade\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eFaster Switching\u003c\/li\u003e\n\u003cli\u003eSteady flow\u003c\/li\u003e\n\u003cli\u003eMicroinjecting\u003c\/li\u003e\n\u003cli\u003eFine microliter delivery\u003c\/li\u003e\n\u003cli\u003eSmall pipette delivery\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eEasy to add to any new or existing gravity perfusion system from AutoMate Scientific, any third-party manufacturer, or even homemade rigs. Connect to house air or compressor (30 to 100 psi). Does not introduce bubbles into solution; helps overcome flow problems due to bubbles.\u003c\/p\u003e\n\u003cp\u003eAvailable in four-, eight- or sixteen-channel configurations. Syringe reservoirs may be placed in a water bath for temperature control. Elegant design allows individual control of each air line.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"fusion-sep-clear\"\u003e \u003c\/div\u003e\n\u003cdiv class=\"fusion-text fusion-text-3\"\u003e\n\u003ch3\u003eOxygen8™\u003c\/h3\u003e\n\u003cp\u003eFor a pressurized perfusion system WITH bubbles, please see our Oxygen8™ system.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"fusion-sep-clear\"\u003e \u003c\/div\u003e\n\u003cdiv class=\"fusion-separator fusion-full-width-sep\"\u003e\n\u003cdiv class=\"fusion-separator-border sep-single sep-dashed\"\u003e \u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"fusion-sep-clear\"\u003e \u003c\/div\u003e\n\u003cdiv class=\"fusion-title title fusion-title-2 fusion-sep-none fusion-title-text fusion-title-size-three\"\u003e\n\u003ch3 class=\"title-heading-left\"\u003eComparison of flow rate change between full and empty reservoirs under gravity and pressure\u003c\/h3\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"fusion-text fusion-text-4\"\u003e\n\u003cp\u003eFull reservoirs always flow faster than empty ones. Researchers doing sensitive electrophysiology or imaging experiments want the flow rate to remain as consistent as possible to minimize capacitance or focus changes over time.\u003c\/p\u003e\n\u003cp\u003eAssumptions in both examples: 60 ml syringe reservoirs mounted 12.5 cm above the perfusion chamber (liquid outlet) with flow constrained to approximately 1 ml\/minute\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eGraph 1: Gravity only – flow rate decreases from 1 ml\/min to 0.7 ml\/min from full (25 cm high) to empty (12.5 cm high)\u003cimg src=\"https:\/\/www.wpi-europe.com\/images\/content\/gravity2.png\" alt=\"\" width=\"650\" height=\"384\"\u003e\n\u003c\/li\u003e\n\u003cli\u003eGraph 2: Pressurized reservoir – flow rate decreases from 1 ml\/min to 0.94 ml\/min from full (25 cm high) to empty (12.5 cm high)\u003cimg src=\"https:\/\/www.wpi-europe.com\/images\/content\/grav3.png\" alt=\"\" width=\"650\" height=\"384\"\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eAdding air pressure decreases the full-to-empty flow rate drop from 30% with gravity to 6% with air pressure for more consistent experiments.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e","products":[],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0662\/7993\/1994\/collections\/PRESSURE2.png?v=1794426905","url":"https:\/\/wpiinc.com\/es\/collections\/pressure-upgrades.oembed","provider":"World Precision Instruments","version":"1.0","type":"link"}