{"title":"Kima","description":"\u003ch1\u003e\u003cspan\u003eKima Microfluidic Pumps\u003c\/span\u003e\u003c\/h1\u003e\n\u003cp\u003e\u003cspan\u003eKima pump is a microfluidic recirculating pump designed to aid cell culture (e.g. endothelial cells) under physiological conditions (shear flow) in various biochips and flow chambers.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg src=\"https:\/\/www.wpi-europe.com\/images\/content\/Kima%20Banner.png\" alt=\"\" width=\"650\" height=\"229\"\u003e\u003c\/p\u003e\n\u003cp\u003eKima pump is a microfluidic recirculating pump controlled by a PC. Suitable for continuous microbe and cell culture under shear flow mimicking physiological flow in the human vasculature. Applications include biofilm studies, cell culture in biochips with adherent cells (HUVECs), stem cells, HepG2 cells.\u003c\/p\u003e\n\u003cp\u003eThe Kima pump is controlled by the KimaFlow control software for use with a PC. The Kima pump can be placed inside a standard incubator. The Kima pump connects easily to Cellix’s Vena8 Endothelial+ biochip and other manufacturer’s flow chambers via fully autoclavable tubing and delivers pulses of fresh media to cells seeded inside the microchannels of the biochip.\u003c\/p\u003e\n\u003cp\u003eThe Kima pump when used with the Vena8 Endothelial+ biochip offers 8 parallel microchannels and with additional Kima pumps the Kima controller can control and run up to 32 microchannels.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eCELL CULTURE UNDER FLOW: simple culture of endothelial cell monolayers under physiological conditions (shear stress) in flow chambers and 8 cell monoalyers in Vena8 Endothelial+ biochips within 24-48 hours\u003c\/li\u003e\n\u003cli\u003eRECIRCULATING CULTURE MEDIA: saves reagents and no additional fluidic valves required\u003c\/li\u003e\n\u003cli\u003eINCUBATOR SAFE: Fits inside all standard CO2  incubators.  No additional temperature or humidity control required\u003c\/li\u003e\n\u003cli\u003ePULSATILE: apply continuous or periodic pulsing flow to cell monolayers in culture\u003c\/li\u003e\n\u003cli\u003ePROGRAMMABLE with iPod Touch\u003c\/li\u003e\n\u003cli\u003eEASY TO USE: control up to 4 pumps with the same controller via PC software KimaFlo control \u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eA Kima pump setup will look like this:\u003c\/p\u003e\n\u003cp\u003e\u003cimg src=\"https:\/\/www.wpi-europe.com\/images\/content\/Kima%20Flow%20Setup.png\" alt=\"\" width=\"650\" height=\"299\"\u003e\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eTechnical Specifications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003ctable border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003ePumping Modes\u003c\/td\u003e\n\u003ctd\u003e2 modes of pumping: wash and perfusion\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eDead Volume\u003c\/td\u003e\n\u003ctd\u003e\u0026lt;  300 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eFlow Rate\u003c\/td\u003e\n\u003ctd\u003ePerfusion mode:  15 - 35 mL\/hr (*) +\/- 4%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eShear Stress Range\u003c\/td\u003e\n\u003ctd\u003ePerfusion mode:  ~2.5 to ~6.5 dyne\/cm2 (**)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eFlow Direction\u003c\/td\u003e\n\u003ctd\u003eDispense only\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ePump Running Conditions\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003eTemperature 10°C–60°C\u003c\/p\u003e\n\u003cp\u003eHumidity \u0026lt;98% RH\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eController\u003c\/td\u003e\n\u003ctd\u003e4 sockets enabling up to 4 Kima pumps to be run independently\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eController Communication\u003c\/td\u003e\n\u003ctd\u003eWi-Fi or USB\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSoftware Control\u003c\/td\u003e\n\u003ctd\u003eKimaFlow software running on Windows PC\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eDimensions of pump\u003c\/td\u003e\n\u003ctd\u003e190 mm (L) x 85 mm (W) x 90 mm (H)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eWeight\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003ePump: 0.8 Kg (excludes bottle and tubing weight)\u003c\/p\u003e\n\u003cp\u003eController: 0.55 Kg\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ePower Requirement\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003eInput: 110\/220V - 50\/60Hz\u003c\/p\u003e\n\u003cp\u003eOutput: 12VDC 3.33A max\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e(*): calibrated test made with a specific flow resistance of 150µm inner diameter by 5cm length\u003c\/p\u003e\n\u003cp\u003e(**): shear stress is for information only; based on the connection to 8 channels of a Vena8 Endothelial+ biochip (capillary of 800µm x 120µm)\u003c\/p\u003e\n\u003cp\u003ePlease Note: the Kima pump is not suitable for shear stressed based assays because the pump delivers liquid in pulses (not continuous flow).\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eCell culture of endothelial cell monolayers for shear flow experiments\u003c\/li\u003e\n\u003cli\u003eCell-cell adhesion shear flow assays on endothelial cell monolayers with Cellix's Mirus Evo Nanopump or ExiGo pump for studies such as:\n\u003cul\u003e\n\u003cli\u003eCardiovascular \/ Atherosclerosis:  primary monocyte adhesion to coronary artery endothelial cells\u003c\/li\u003e\n\u003cli\u003eInflammation:  THP-1 monocyte adhesion to HUVECs under flow\u003c\/li\u003e\n\u003cli\u003eAsthma \/ COPD: eosinophil adhesion studies to human lung microvascular endothelial cells (HMVEC-L)\u003c\/li\u003e\n\u003cli\u003eOncology - Breast Cancer:  MDA cells adhering to TNF-alpha stimulated HUVECs under shear flow\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eKima Pump Videos\u003c\/h2\u003e\n\u003cp\u003e\u003ciframe src=\"https:\/\/www.youtube.com\/embed\/NLjcmpnB6NU\" width=\"300\" height=\"168\"\u003e\u003c\/iframe\u003e \u003ciframe src=\"https:\/\/www.youtube.com\/embed\/fO1AKhckz1s\" width=\"300\" height=\"168\"\u003e\u003c\/iframe\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ciframe src=\"https:\/\/www.youtube.com\/embed\/45_JaP85Wog\" width=\"300\" height=\"168\"\u003e\u003c\/iframe\u003e \u003ciframe src=\"https:\/\/www.youtube.com\/embed\/M0OS_gvc660\" width=\"300\" height=\"168\"\u003e\u003c\/iframe\u003e\u003c\/p\u003e","products":[],"url":"https:\/\/wpiinc.com\/collections\/kima.oembed","provider":"World Precision Instruments","version":"1.0","type":"link"}